Optical Fingerprinting in Bacterial Epidemiology: Raman Spectroscopy as a Real-Time Typing Method
- 1 March 2009
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 47 (3), 652-659
- https://doi.org/10.1128/jcm.01900-08
Abstract
Hospital-acquired infections (HAI) increase morbidity and mortality and constitute a high financial burden on health care systems. An effective weapon against HAI is early detection of potential outbreaks and sources of contamination. Such monitoring requires microbial typing with sufficient reproducibility and discriminatory power. Here, a microbial-typing method is presented, based on Raman spectroscopy. This technique provides strain-specific optical fingerprints in a few minutes instead of several hours to days, as is the case with genotyping methods. Although the method is generally applicable, we used 118 Staphylococcus aureus isolates to illustrate that the discriminatory power matches that of established genotyping techniques (numerical index of diversity [ D ] = 0.989) and that concordance with the gold standard (pulsed-field gel electrophoresis) is high (95%). The Raman clustering of isolates was reproducible to the strain level for five independent cultures, despite the various culture times from 18 h to 24 h. Furthermore, this technique was able to classify stored (−80°C) and recent isolates of a methicillin-resistant Staphylococcus aureus -colonized individual during surveillance studies and did so days earlier than established genotyping techniques did. Its high throughput and ease of use make it suitable for use in routine diagnostic laboratory settings. This will set the stage for continuous, automated, real-time epidemiological monitoring of bacterial infections in a hospital, which can then be followed by timely corrective action by infection prevention teams.Keywords
This publication has 26 references indexed in Scilit:
- A novel approach to correct variations in Raman spectra due to photo-bleachable cellular componentsThe Analyst, 2008
- Molecular Characterization of Staphylococcus aureus Bloodstream Isolates Collected in a Dutch University Hospital between 1999 and 2006Journal of Clinical Microbiology, 2008
- Daily cost of antimicrobial therapy in patients with Intensive Care Unit-acquired, laboratory-confirmed bloodstream infectionInternational Journal of Antimicrobial Agents, 2008
- Raising Standards While Watching the Bottom Line Making a Business Case for Infection ControlInfection Control & Hospital Epidemiology, 2007
- Evaluation of Molecular Typing Methods in Characterizing a European Collection of Epidemic Methicillin-Resistant Staphylococcus aureus Strains: the HARMONY CollectionJournal of Clinical Microbiology, 2007
- Current Trends in the Epidemiological typing of clinically relevant microbes in EuropeJournal of Microbiological Methods, 2007
- Costs of hospital-acquired methicillin-resistant Staphylococcus aureus (MRSA) and its controlInternational Journal of Antimicrobial Agents, 2006
- Rapid epidemiological analysis of Acinetobacter strains by Raman spectroscopyJournal of Microbiological Methods, 2006
- Binary Typing of Staphylococcus aureus Strains through Reversed Hybridization Using Digoxigenin-Universal Linkage System-Labeled Bacterial Genomic DNAJournal of Clinical Microbiology, 2001
- Extended multiplicative signal correction and spectral interference subtraction: New preprocessing methods for near infrared spectroscopyJournal of Pharmaceutical and Biomedical Analysis, 1991