Use of electrospray mass spectrometry to directly observe an acyl enzyme intermediate in β‐lactamase catalysis
Open Access
- 17 December 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 277 (1-2), 212-214
- https://doi.org/10.1016/0014-5793(90)80847-c
Abstract
Electrospray mass spectrometry was used to directly observe intact acyl enzyme complexes formed between a class C β‐lactamase (from Enterobacter cloacae P99) and four poor substrates/inhibitors. In each case the molecular weight difference between the unreacted and the reacted β‐lactamase was consistent with the formation of an acyl enzyme.Keywords
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