IN VIVO IMAGING OF LYMPHOCYTE TRAFFICKING
- 1 November 2005
- journal article
- review article
- Published by Annual Reviews in Annual Review of Cell and Developmental Biology
- Vol. 21 (1), 581-603
- https://doi.org/10.1146/annurev.cellbio.21.122303.133159
Abstract
Over the past decades, intravital microscopy (IVM), the imaging of cells in living organisms, has become a valuable tool for studying the molecular determinants of lymphocyte trafficking. Recent advances in microscopy now make it possible to image cell migration and cell-cell interactions in vivo deep within intact tissues. Here, we summarize the principal techniques that are currently used in IVM, discuss options and tools for fluorescence-based visualization of lymphocytes in microvessels and tissues, and describe IVM models used to explore lymphoid and non-lymphoid organs. The latter will be introduced according to the physiologic itinerary of developing and differentiating T and B lymphocytes as they traffic through the body, beginning with their development in bone marrow and thymus and continuing with their migration to secondary lymphoid organs and peripheral tissues.Keywords
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