A yeast ARS-binding protein activates transcription synergistically in combination with other weak activating factors.
Open Access
- 1 March 1990
- journal article
- research article
- Published by Informa UK Limited in Molecular and Cellular Biology
- Vol. 10 (3), 887-897
- https://doi.org/10.1128/mcb.10.3.887
Abstract
ABFI (ARS-binding protein I) is a yeast protein that binds specific DNA sequences associated with several autonomously replicating sequences (ARSs). ABFI also binds sequences located in promoter regions of some yeast genes, including DED1, an essential gene of unknown function that is transcribed constitutively at a high level. ABFI was purified by specific binding to the DED1 upstream activating sequence (UAS) and was found to recognize related sequences at several other promoters, at an ARS (ARS1), and at a transcriptional silencer (HMR E). All ABFI-binding sites, regardless of origin, provided weak UAS function in vivo when examined in test plasmids. UAS function was abolished by point mutations that reduced ABFI binding in vitro. Analysis of the DED1 promoter showed that two ABFI-binding sites combine synergistically with an adjacent T-rich sequence to form a strong constitutive activator. The DED1 T-rich element acted synergistically with all other ABFI-binding sites and with binding sites for other multifunctional yeast activators. An examination of the properties of sequences surrounding ARS1 left open the possibility that ABFI enhances the initiation of DNA replication at ARS1 by transcriptional activation.This publication has 61 references indexed in Scilit:
- Statistical positioning of nucleosomes by specific protein-binding to an upstream activating sequence in yeastJournal of Molecular Biology, 1988
- Transcriptional activation of initiation of replication from the E. coli chromosomal origin: An RNA-DNA hybrid near oriCCell, 1988
- A yeast silencer contains sequences that can promote autonomous plasmid replication and transcriptional activationCell, 1987
- A common octamer motif binding protein is involved in the transcription of U6 snRNA by RNA polymerase III and U2 snRNA by RNA polymerase IICell, 1987
- Nuclear factor III, a novel sequence-specific DNA-binding protein from HeLa cells stimulating adenovirus DNA replicationNature, 1986
- The SV40 enhancer is composed of multiple functional elements that can compensate for one anotherCell, 1986
- Transcription initiation of the Saccharomyces cerevisiae iso-1-cytochrome c geneJournal of Molecular Biology, 1986
- A region flanking the GAL7 gene and a binding site for GAL4 protein as upstream activating sequences in yeastJournal of Molecular Biology, 1985
- Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box regionCell, 1985
- Centromeric DNA from Saccharomyces cerevisiaeJournal of Molecular Biology, 1982