Fetal membrane collagens: identification of two new collagen alpha chains.
- 1 August 1976
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 73 (8), 2579-2583
- https://doi.org/10.1073/pnas.73.8.2579
Abstract
Human fetal membranes contain 2 new genetically distinct collagen polypeptide chains which are subunits of 1 (or 2) new molecular species of collagen. These new polypeptide chains, which were tentatively named .alpha.A and .alpha.B, were directly compared with the polypeptide chain subunits of Types I, II, and III human collagen and Type IV collagen from bovine lens capsule. Both .alpha.A and .alpha.B exhibit characteristic profiles on carboxymethyl-cellulose chromatography and sodium dodecyl sulfate/polyacrylamide gel electrophoresis [SDS-PAGE]. The distribution of methionine residues along both new chains is different from known collagen chains as manifest by distinctly different cyanogen bromide peptide profiles on carboxymethyl-cellulose chromatography and/or SDS-PAGE. Both .alpha.A and .alpha.B exhibit content of imino acids and glycine typical of collagens, and comparison with the observed and reported compositions of collagen chains of Types I-IV collagens reveals notable differences, particularly in the content of alanine, leucine, isoleucine, and the basic amino acids, lysine, hydroxylysine, and arginine. The new collagen species containing both .alpha.A and .alpha.B may be separated in the native (triple-helical) state from other native collagen species by differential salt precipitation. The observations that both chains coprecipitate in the same narrow NaCl range, and that the ratio of .alpha.A:.alpha.B is constant, suggest the possibility of a single new species of collagen with a subunit structure .alpha.A[.alpha.B]2.This publication has 29 references indexed in Scilit:
- Studies on matrix proteins of normal and lathyritic rat bone and dentineCalcified Tissue International, 1974
- Structural studies on cartilage collagen employing limited cleavage and solubilization with pepsinBiochemistry, 1972
- Collagen synthesis by cells II: Secretion of a disulfide linked materialBiochemical and Biophysical Research Communications, 1972
- Isolation and characterization of cyanogen bromide peptides from basement membrane collagenBiochemical and Biophysical Research Communications, 1972
- Isolation of a collagen from basement membranes containing three identical α-chainsBiochemical and Biophysical Research Communications, 1971
- Isolation and characterization of a chick cartilage collagen containing three identical chainsBiochemistry, 1971
- Use of a mixture of proteinase-free collagenases for the specific assay of radioactive collagen in the presence of other proteinsBiochemistry, 1971
- Isolation of two distinct collagens from chick cartilageBiochemistry, 1970
- Isolation and characterization of the cyanogen bromide peptides from the α1 and α2 chains of human skin collagenBiochemistry, 1970
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970