A sulfate‐reducing bacterium that can detoxify U(VI) and obtain energy via nitrate reduction
- 15 July 2003
- journal article
- research article
- Published by Wiley in Journal of Basic Microbiology
- Vol. 43 (4), 348-361
- https://doi.org/10.1002/jobm.200390038
Abstract
Bacterial strain UFZ B 490, which was isolated from a uranium dump and is closely related to Desulfovibrio vulgaris oxamicusT (DSM 1925T), is able to detoxify U(VI) in aqueous media. In experiments reported here, U(VI) was used as an electron acceptor and lactate as electron donor. The reduction of soluble U(VI) to solid U(IV) (uraninite) did not provide energy for growth of strain UFZ B 490. However, the isolate is able to grow when supplied with nitrate as sole electron acceptor and nitrogen source, using lactate as a source of carbon and energy. In comparative studies, the strains Desulfovibrio vulgaris oxamicusT (DSM 1925T) and Desulfovibrio vulgaris vulgarisT (DSM 644T), as well as the isolate, all utilized 0.6 mol lactate per mol U(VI) reduced. Strains UFZ B 490 and Desulfovibrio vulgaris oxamicusT (DSM 1925T) were found to consume 2.4 mol lactate per mol nitrate reduced, but Desulfovibrio vulgaris vulgarisT (DSM 644T) did not display dissimilatory nitrate reduction. In further experiments it was found that strain UFZ B 490 preferred sulfate as electron acceptor in the presence of both sulfate and nitrate, irrespective of whether it had been precultivated on sulfate or nitrate.Keywords
This publication has 38 references indexed in Scilit:
- Technetium reduction and precipitation by sulfate‐reducing bacteriaGeomicrobiology Journal, 1998
- Impact of organic ligands on uranium removal during anaerobic biological treatmentWater Science & Technology, 1998
- A common pathway of sulfide oxidation by sulfate-reducing bacteriaFEMS Microbiology Letters, 1996
- Isolation and characterization of a sulfate-reducing bacterium that removed TNT (2,4,6-trinitrotoluene) under sulfate- and nitrate-reducing conditionsBioresource Technology, 1996
- The preferred electron acceptor of Desulfovibrio desulfuricans CSNFEMS Microbiology Ecology, 1995
- Characterization of a methanol‐utilizing sulfate‐reducing bacterium isolated from a wastewater pondJournal of Basic Microbiology, 1995
- DISSIMILATORY METAL REDUCTIONAnnual Review of Microbiology, 1993
- Aerobic respiration in sulfate-reducing bacteria*FEMS Microbiology Letters, 1990
- Dissimilatory nitrate reduction by a strain ofDesulfovibrio desulfuricansFEMS Microbiology Letters, 1983
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976