Tempol, a Nitroxide Antioxidant, Improves Locomotor and Histological Outcomes after Spinal Cord Contusion in Rats
- 1 October 2004
- journal article
- research article
- Published by Mary Ann Liebert Inc in Journal of Neurotrauma
- Vol. 21 (10), 1405-1414
- https://doi.org/10.1089/neu.2004.21.1405
Abstract
We have determined whether the nitroxide antioxidant, tempol, can oppose tissue loss and improve recovery of locomotor function following contusion injury of the spinal cord. Contusion injury was produced in rats at the level of T10 with a weight-drop device and locomotor recovery was determined with the 21-point Basso, Beattie and Bresnahan (BBB) scale. Locomotor function recovered progressively during the 6–week postinjury observation period and was significantly greater in tempol- treated (275 mg/kg ip, 20 min postinjury) compared to vehicle-treated rats (final BBB scores: 9.1 versus 6.4). Similarly enhanced locomotor recovery was observed with doses of tempol in the range of 138–550, but not 69 mg/kg, and with injection at 48 h postinjury indicating a therapeutic time-window of at least several days. The extent of recovery correlated with measurements of sparing of spinal cord white matter in a region several millimeters in length extending rostrally from the contusion epicenter. In contrast, loss of gray matter was unaffected by tempol treatment. Since tempol acts by scavenging reactive oxygen species (ROS) such as superoxide and hydroxyl radicals, the improved locomotor recovery and spared spinal cord tissue following contusion injury provides evidence of a direct role of ROS-mediated neurodegeneration in spinal cord injury.Keywords
This publication has 39 references indexed in Scilit:
- Quercetin Promotes Functional Recovery Following Acute Spinal Cord InjuryJournal of Neurotrauma, 2003
- Tempol, a Novel Stable Nitroxide, Reduces Brain Damage and Free Radical Production, after Acute Subdural Hematoma in the RatJournal of Neurotrauma, 2003
- Neuroprotection by the stable nitroxide 3-carbamoyl-proxyl during reperfusion in a rat model of transient focal ischemiaJournal of Neurosurgery, 2003
- Methylprednisolone and Interleukin-10 Reduce Gray Matter Damage in the Contused Fischer Rat Thoracic Spinal Cord but Do Not Improve Functional OutcomeJournal of Neurotrauma, 2002
- X-irradiation of the Contusion Site Improves Locomotor and Histological Outcomes in Spinal Cord-Injured RatsExperimental Neurology, 2001
- Promoting glutathione synthesis after spinal cord trauma decreases secondary damage and promotes retention of functionThe FASEB Journal, 2000
- Basic Fibroblast Growth Factor (bFGF) Enhances Functional Recovery Following Severe Spinal Cord Injury to the RatExperimental Neurology, 2000
- Neuroprotection by the stable nitroxide Tempol during reperfusion in a rat model of transient focal ischemiaJournal of Neurosurgery, 2000
- Clenbuterol, a β2-Adrenoceptor Agonist, Improves Locomotor and Histological Outcomes after Spinal Cord Contusion in RatsExperimental Neurology, 1999
- A Sensitive and Reliable Locomotor Rating Scale for Open Field Testing in RatsJournal of Neurotrauma, 1995