Development of Murine Hepatic NK Cells during Ontogeny: Comparison with Spleen NK Cells
Open Access
- 1 January 2012
- journal article
- Published by Hindawi Limited in Clinical and Developmental Immunology
- Vol. 2012, 1-12
- https://doi.org/10.1155/2012/759765
Abstract
The phenotype of developing liver NK cells (CD3−NK1.1+) was investigated during mouse ontogeny comparing with spleen NK cells. The highest percentage of hepatic CD27−CD11b−NK cells occurred at the fetal stage. After birth, the percentage of CD27−CD11b−NK cells in both the liver and spleen gradually decreased to their lowest level at 6 weeks. More CD27+CD11b−NK cells were detected in the liver than that in spleen from week 1 to 6. Expression of NKG2A on liver NK cells was decreased but still much higher than that of spleen NK cells after 1 week. The NKG2D expression on liver NK cells increased to its highest level and was significantly higher than on spleen NK cells till 4 weeks. During mouse ontogeny, weaker expression of NKp46 and CD2 and stronger expression of CD69, CD11c, 2B4, and CD73 were observed on liver NK cells. Furthermore, neonatal liver NK cells express higher IFN-γ and perforin than adult .These results suggest that the maturation process of NK cells is unique in the livers, and liver microenvironments might play critical roles to keep NK cells in an immature status.Keywords
Funding Information
- National Natural Science Foundation of China (30721002, 30870998, 30801006, 2007CB512807, 2010CB945303)
This publication has 37 references indexed in Scilit:
- Intrahepatic IL-10 Maintains NKG2A+Ly49− Liver NK Cells in a Functionally Hyporesponsive StatePublished by The American Association of Immunologists ,2010
- Unique features of NK cell development during ontogeny revealed in studies of RAG‐1‐deficient miceImmunology & Cell Biology, 2009
- A potential role for RAG‐1 in NK cell development revealed by analysis of NK cells during ontogenyImmunology & Cell Biology, 2009
- Maturation of mouse NK cells is a 4-stage developmental programBlood, 2009
- CD11c identifies a subset of murine liver natural killer cells that responds to adenoviral hepatitisJournal of Leukocyte Biology, 2008
- The activation state of human intrahepatic lymphocytesClinical and Experimental Immunology, 2007
- TRAIL identifies immature natural killer cells in newborn mice and adult mouse liverBlood, 2005
- What does it take to make a natural killer?Nature Reviews Immunology, 2003
- Anatomical basis of tolerance and immunity to intestinal antigensNature Reviews Immunology, 2003
- In vivo developmental stages in murine natural killer cell maturationNature Immunology, 2002