In vitroeffects of beetroot juice and chips on oxidative metabolism and apoptosis in neutrophils from obese individuals
Open Access
- 23 September 2008
- journal article
- research article
- Published by Wiley in Phytotherapy Research
- Vol. 23 (1), 49-55
- https://doi.org/10.1002/ptr.2535
Abstract
Oxidative stress and inflammation are involved in the development of obesity. Beetroot (Beta vulgaris var. rubra) is a food ingredient containing betalain pigments that show antioxidant activity. The in vitro effect of beetroot juice and chips on oxidative metabolism and apoptosis in neutrophils from obese individuals has been investigated. Fifteen obese women (aged 45 ± 9 years, BMI >30 kg/m2) and nine healthy controls (women, aged 29 ± 11 years, BMI = 22.2 ± 1.6 kg/m2) were examined. The investigated products were used as concentrates and after transport and digestion in an artificial gastrointestinal tract. Neutrophil oxidant production, in response to phorbol 12‐myristate 13‐acetate, was characterized by luminol‐dependent chemiluminescence and a flow cytometric dichlorofluorescin oxidation assay. Caspase‐3 activity, a marker of apoptosis, was measured by cleavage of the fluorogenic substrate Ac‐DEVD‐AMC. Neutrophils from obese individuals had a significantly higher ROS production compared with the controls (p < 0.05). Beetroot products inhibited neutrophil oxidative metabolism in a concentration‐dependent manner. Also observed were the pro‐apoptotic effects of beetroot at a concentration range of 0.1–10% in 24 h culture of stimulated neutrophils. These natural products (in both the liquid and solid state) have antioxidant and antiinflammatory capacity, and could be an important adjunct in the treatment of obesity. Copyright © 2008 John Wiley & Sons, Ltd.Keywords
This publication has 34 references indexed in Scilit:
- A Functional NADPH Oxidase Prevents Caspase Involvement in the Clearance of Phagocytic NeutrophilsInfection and Immunity, 2007
- Anin vitro study of the protective effect of the flavonoid silydianin against reactive oxygen speciesPhytotherapy Research, 2006
- Mechanism of interaction of betanin and indicaxanthin with human myeloperoxidase and hypochlorous acidBiochemical and Biophysical Research Communications, 2005
- Superoxide dismutase (SOD) as a potential inhibitory mediator of inflammation via neutrophil apoptosisFree Radical Research, 2005
- Apoptosis of NeutrophilsActa Haematologica, 2003
- Obesity and Systemic Oxidative StressArteriosclerosis, Thrombosis, and Vascular Biology, 2003
- Obesity and Oxidative StressArteriosclerosis, Thrombosis, and Vascular Biology, 2003
- The central roles of obesity-associated dyslipidaemia, endothelial activation and cytokines in the Metabolic Syndrome—an analysis by structural equation modellingInternational Journal of Obesity, 2002
- Antioxidant Activity of Plant Extracts Containing Phenolic CompoundsJournal of Agricultural and Food Chemistry, 1999
- Chemoprevention of lung and skin cancer by Beta vulgaris (beet) root extractCancer Letters, 1996