Enumeration of an Extremely High Particle-to-PFU Ratio for Varicella-Zoster Virus
- 1 July 2009
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 83 (13), 6917-6921
- https://doi.org/10.1128/jvi.00081-09
Abstract
Varicella-zoster virus (VZV) is renowned for its low titers. Yet investigations to explore the low infectivity are hampered by the fact that the VZV particle-to-PFU ratio has never been determined with precision. Herein, we accomplish that task by applying newer imaging technology. More than 300 images were taken of VZV-infected cells on 4 different samples at high magnification. We enumerated the total number of viral particles within 25 cm2of the infected monolayer at 415 million. Based on these numbers, the VZV particle:PFU ratio was approximately 40,000:1 for a cell-free inoculum.Keywords
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