Determination of zearalenone and ochratoxin a mycotoxins in grain by fluorescence polarization immunoassay

Abstract

Rapid procedures are reported for the determination of zearalenone and ochratoxin A, which make possible the analysis of up to 10 grain samples within 20 min by fluorescence polarization immunoassay. The influence of various fluorescein-based tracers on the sensitivity of analysis is examined. The most promising was admitted the use of ethylenediamine thiocarbamoylfluorescein and aminomethylfluorescein. The detection limits of the method for zearalenone and ochratoxin A were 3 and 1.5 ng/mL for a model system and 15 and 10 μg/kg for grain, respectively. The performed analysis of spiked grain samples demonstrated the recovery of mycotoxins at a level of 84–97%.