Scanning microscopy of dissociated tissue cells.
Open Access
- 1 November 1975
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 67 (2), 345-360
- https://doi.org/10.1083/jcb.67.2.345
Abstract
A method is described for studying by scanning electron microscopy (SEM) all the surfaces of fully differentiated cells from intact tissues. Thus, cell faces normally hidden from view are exposed and made available for SEM examination. This is achieved by fixing the tissue in OSO4 and then soaking it in a 1% solution (in water) of boric acid. After different periods of time, varied according to particular tissue, slight mechanical pressure will cause the fixed tissue to dissociate into its component cells. These are then made to adhere to a substrate and are taken through critical point drying, etc., for examination. Observations are reported on the topography of whole hepatocytes, adsorptive cells of the intestinal epithelium, proximal tubule cells of the rat kidney, mammary tumor cells of the mouse, and rat sarcoma cells. Several other tissues are reported to dissociate when similarly treated, but for each the procedure must be slightly modified.Keywords
This publication has 12 references indexed in Scilit:
- A scanning electron microscopic study of the nephronJournal of Anatomy, 1974
- Structure of rat liver sinusoids and associated tissue spaces as revealed by scanning electron microscopyCell and tissue research, 1974
- Membrane alterations which accompany MuMTV maturationVirology, 1974
- A SCANNING ELECTRON MICROSCOPE STUDY OF SURFACE FEATURES OF VIRAL AND SPONTANEOUS TRANSFORMANTS OF MOUSE BALB/3T3 CELLSThe Journal of cell biology, 1973
- The ultrastructural morphology and possible functional significance of mesothelial microvilliThe Anatomical Record, 1973
- Scanning electron microscopy of rat's liver. Application of freeze-fracture and freeze-drying techniques.1973
- Cell Surface Movements Related to Cell LocomotionPublished by Wiley ,1973
- Standing-Gradient Osmotic FlowThe Journal of general physiology, 1967
- INTESTINAL TRIGLYCERIDE ABSORPTION IN THE RATThe Journal of cell biology, 1967
- Studies on the Fine Structure of the Terminal Branches of the Biliary Tree: I. The Morphology of Normal Bile Canaliculi, Bile Pre-ductules (Ducts of Hering) and Bile Ductules.1961