Combined reflectance and fluorescence spectroscopy for in vivo detection of cervical pre-cancer
- 1 January 2005
- journal article
- research article
- Published by SPIE-Intl Soc Optical Eng in Journal of Biomedical Optics
- Vol. 10 (2), 024031
- https://doi.org/10.1117/1.1899686
Abstract
Optical technologies, such as reflectance and fluorescence spectroscopy, have shown the potential to provide improved point-of-care detection methods for cervical neoplasia that are sensitive, specific, and cost-effective. Our specific goals are to analyze the diagnostic potential of reflectance and fluorescence spectra, alone and in combination, to discriminate normal and precancerous cervical tissue in vivo and to identify which classification features contain significant diagnostic information. Reflectance spectra are measured at four source-detector separations and fluorescence emission spectra are measured at 16 excitation wavelengths, from 324 sites in 161 patients. These 20 spectral features are permuted in all possible combinations of one, two, and three; and classification algorithms are developed to evaluate the diagnostic performance of each combination. Algorithms based on fluorescence spectra alone yield better diagnostic performance than those based on reflectance spectra alone. The combination of fluorescence and reflectance do not significantly improve diagnostic performance compared to fluorescence alone, except in the case of discriminating high-grade precancers from columnar normal tissue. In general, fluorescence emission spectra at 330- to 360-nm and 460- to 470-nm excitation provide the best diagnostic performance for separating all pairs of tissue categories.Keywords
This publication has 22 references indexed in Scilit:
- Estimates of the worldwide mortality from 25 cancers in 1990International Journal of Cancer, 1999
- Measurement of tissue optical properties by the use of oblique-incidence optical fiber reflectometryApplied Optics, 1997
- Cervical Precancer Detection Using a Multivariate Statistical Algorithm Based on Laser‐Induced Fluorescence Spectra at Multiple Excitation WavelengthsPhotochemistry and Photobiology, 1996
- Detection of dysplasia at colonoscopy using laser-induced fluorescence: a blinded studyGastrointestinal Endoscopy, 1996
- Influence of the scattering phase function on light transport measurements in turbid media performed with small source–detector separationsOptics Letters, 1996
- Meta-analysis of Pap Test AccuracyAmerican Journal of Epidemiology, 1995
- In vivo cancer diagnosis of the esophagus using differential normalized fluorescence (DNF) indicesLasers in Surgery and Medicine, 1995
- Spectroscopic diagnosis of bladder cancer with elastic light scatteringLasers in Surgery and Medicine, 1995
- An electronic approach to the detection of pre-cancer and cancer of the uterine cervix: a preliminary evaluation of PolarprobeInternational Journal of Gynecologic Cancer, 1994
- Fluorescence spectra from cancerous and normal human breast and lung tissuesIEEE Journal of Quantum Electronics, 1987