Colorimetric Method for the Determination of Lipoxygenase Activity
- 6 December 2000
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 49 (1), 32-37
- https://doi.org/10.1021/jf000871s
Abstract
A colorimetic assay for lipoxygenase activity has been developed. The assay is based on the detection of the lipoxygenase reaction product, linoleic acid hydroperoxide, by the oxidative coupling of 3-methyl-2-benzothiazolinone (MBTH) with 3-(dimethylamino)benzoic acid (DMAB) in a hemoglobin-catalyzed reaction. This test reaction is rapid and sensitive, and it offers advantages over other methods for detecting lipoxygenase activity. The assay is capable of detecting activity in a number of crude vegetable homogenates and should be particularly useful where a rapid visual determination of lipoxygenase activity is desired. Keywords: Lipoxygenase; colorimetric assay; rapid; vegetable homogenates; hemoglobinKeywords
This publication has 11 references indexed in Scilit:
- Potato lipoxygenase catalysed co-oxidation of β-caroteneFood Chemistry, 1999
- Rapid Methods for Lipoxygenase Assay in Sweet CornJournal of Food Science, 1997
- A Colorimetric Method for the Determination of Lipoxygenase Activity Suitable for Use in a High Throughput Assay FormatAnalytical Biochemistry, 1995
- Simple and Rapid Method for the Selective Detection of Individual Lipoxygenase Isoenzymes in Soybean SeedsJournal of Agricultural and Food Chemistry, 1995
- Kinetic analysis of the induction period in lipoxygenase catalysisBiochemistry, 1993
- Bleaching of Methylene Blue as an Index of Lipoxygenase ActivityPublished by Oxford University Press (OUP) ,1992
- A spectrophotometric microtiter-based assay for the detection of hydroperoxy derivatives of linoleic acidAnalytical Biochemistry, 1992
- Product yield in oxygenation of linoleate by soybean lipoxygenase: The value of the molar extinction coefficient in the spectrophotometric assayAnalytical Biochemistry, 1987
- Detection of picomole levels of hydroperoxides using a fluorescent dichlorofluorescein assayAnalytical Biochemistry, 1983
- Measurement of lipoxygenase activity in crude and partially purified potato extractsPhytochemistry, 1976