Fgf-8 expression in the post-gastrulation mouse suggests roles in the development of the face, limbs and central nervous system
- 1 November 1994
- journal article
- Published by Elsevier BV in Mechanisms of Development
- Vol. 48 (2), 129-138
- https://doi.org/10.1016/0925-4773(94)90022-1
Abstract
Fgf-8 is a member of the fibroblast growth factor (FGF) family that was initially identified as an androgen-inducible growth factor in a mammary carcinoma cell line. Alternative splicing of the primary Fgf-8 transcript results in three messenger RNAs which code for secreted FGF-8 protein isoforms that differ only in their mature amino termini. Fgf-8 RNA is present from day 10 through 12 of murine gestation when analyzed by northern blot analysis, suggesting that Fgf-8 normally functions during post-gastrulation development. To characterize the temporal, spatial and isoform-specific aspects of Fgf-8 expression during mouse development, we performed in situ hybrization and ribonuclease protection assays between the days 8 and 16 of gestation. Fgf-8 expression is first detected at day 9 of gestation in the surface ectoderm of the first branchial arches, the frontonasal process, the forebrain and the midbrain-hindbrain junction. At days 10–12 of gestation, Fgf-8 expression is detected in the surface ectoderm of the forelimb and hindlimb buds, in the nasal pits and nasopharynx, in the infundibulum and in the telecephalon, diencephalon and metencephalon. Fgf-8 expression continues in the developing hindlimbs through day 13 of gestation but is undetectable thereafter. Ribonuclease protection assays reveal that RNAs coding for all three FGF-8 isoforms are present at days 10–12 of gestation. These results reveal a unique temporal and spatial pattern of Fgf-8 expression in the developing mouse and suggest a role for this FGF in multiple regions of ectodermal differentiation in the post-gastrulation mouse embryo.Keywords
This publication has 34 references indexed in Scilit:
- FGF-7 (keratinocyte growth factor) expression during mouse development suggests roles in myogenesis, forebrain regionalisation and epithelial-mesenchymal interactionsMechanisms of Development, 1994
- Embryonic Expression of Fgf-6 Is Restricted to the Skeletal Muscle LineageDevelopmental Biology, 1993
- Murine FGF-4 gene expression is spatially restricted within embryonic skeletal muscle and other tissuesMechanisms of Development, 1993
- Identification of a cysteine-rich receptor for fibroblast growth factors.Molecular and Cellular Biology, 1992
- Responses of embryonic xenopus cells to activin and FGF are separated by multiple dose thresholds and correspond to distinct axes of the mesodermCell, 1992
- The Fgf Family of Growth Factors and OncogenesAdvances in Cancer Research, 1992
- A dual receptor system is required for basic fibroblast growth factor activityCell, 1991
- Expression of a dominant negative mutant of the FGF receptor disrupts mesoderm formation in xenopus embryosCell, 1991
- Synergistic induction of mesoderm by FGF and TGF-β and the identification of an mRNA coding for FGF in the early xenopus embryoCell, 1987
- Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extractionAnalytical Biochemistry, 1987