Colorimetric Bio-Barcode Amplification Assay for Cytokines
- 1 October 2005
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 77 (21), 6985-6988
- https://doi.org/10.1021/ac0513764
Abstract
The bio-barcode amplification assay has become a powerful tool in detecting tens to hundreds of biological targets such as proteins and nucleic acids in the entire sample. However, current bio-barcode detection schemes still require many experimental steps including microarrayer-based immobilization of oligonucleotides on a glass chip, silver enhancement of immobilized gold nanoparticles on a chip, and light-scattering measurement. Here, we report a colorimetric bio-barcode method that minimizes the above requirements while detecting 30 aM concentrations of cytokines (∼3 orders of magnitude more sensitive than conventional nonenzymatic cytokine detection assays). The assay is based on porous microparticles, which enable loading of a large number of barcode DNA per particle, and gold nanoparticle-based colorimetric barcode detection method.Keywords
This publication has 20 references indexed in Scilit:
- Nanostructures in BiodiagnosticsChemical Reviews, 2005
- DNA‐Based Amplified Bioelectronic Detection and Coding of ProteinsAngewandte Chemie-International Edition, 2004
- Particle Formation by Ion Nucleation in the Upper Troposphere and Lower StratosphereScience, 2003
- Fluidity of Bound Hydration LayersScience, 2002
- Multiplexed protein profiling on microarrays by rolling-circle amplificationNature Biotechnology, 2002
- Oligonucleotide Adsorption to Gold Nanoparticles: A Surface-Enhanced Raman Spectroscopy Study of Intrinsically Bent DNAThe Journal of Physical Chemistry B, 2001
- Stable Ordering in Langmuir-Blodgett FilmsScience, 2001
- Encapsulation, Permeability, and Cellular Uptake Characteristics of Hollow Nanometer-Sized Conductive Polymer CapsulesThe Journal of Physical Chemistry B, 2001
- One-Pot Colorimetric Differentiation of Polynucleotides with Single Base Imperfections Using Gold Nanoparticle ProbesJournal of the American Chemical Society, 1998
- Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell AnemiaScience, 1985