Protein and mucin retention on oral mucosal surfaces in dry mouth patients
- 11 May 2010
- journal article
- research article
- Published by Wiley in European Journal of Oral Sciences
- Vol. 118 (3), 245-253
- https://doi.org/10.1111/j.1600-0722.2010.00728.x
Abstract
Oral homeostasis depends largely on proteins and mucins present in saliva that coat all oral surfaces. The present study compared the protein composition of residual fluid on mucosal surfaces in subjects with normal salivary flow with that of patients with dry mouth caused by salivary hypofunction. Samples of residual mucosal fluid were collected using paper strips and then analysed by protein electrophoresis and immunoblotting. In both patients and controls, residual fluids on mucosal surfaces (except the anterior tongue in control subjects) had higher protein concentrations than unstimulated whole-mouth saliva. High-molecular-weight mucin (MUC5B) was present in greater amounts on the anterior tongue than on other surfaces in control subjects. In dry mouth patients who were unable to provide a measurable saliva sample, MUC5B was often still present on all mucosal surfaces but in reduced amounts on the anterior tongue. The membrane-bound mucin, MUC1, was prominent on buccal and labial surfaces in patients and controls. Statherin was still present on surfaces that were dried to remove salivary fluid, suggesting that it may be adsorbed as a protein pellicle. It is concluded that oral mucosal surfaces in dry mouth patients can retain MUC5B and other salivary proteins, although the functional integrity of these proteins is uncertain.Keywords
This publication has 30 references indexed in Scilit:
- Structure and Function of the Polymeric Mucins in Airways MucusAnnual Review of Physiology, 2008
- MUC1 cell surface mucin is a critical element of the mucosal barrier to infectionJCI Insight, 2007
- Comparison of the composition of oral mucosal residual saliva with whole salivaOral Diseases, 2007
- Salivary Proteome and Its Genetic PolymorphismsAnnals of the New York Academy of Sciences, 2007
- A statherin and calcium enriched layer at the air interface of human parotid salivaBiochemical Journal, 2005
- New Monoclonal Antibodies to Non-Glycosylated Domains of the Secreted Mucins MUC5B and MUC7Hybridoma and Hybridomics, 2003
- Detection and Quantification of MUC7 in Submandibular, Sublingual, Palatine, and Labial Saliva by Anti-peptide AntiserumJournal of Dental Research, 1999
- Detection of a Secreted MUC1/SEC Protein by MUC1 Isoform Specific Monoclonal AntibodiesBiochemical and Biophysical Research Communications, 1996
- Statherin: A major boundary lubricant of human salivaBiochemical and Biophysical Research Communications, 1991
- A Mathematical Model of Salivary Clearance of Sugar from the Oral CavityCaries Research, 1983