Characterization of the Bacillus subtilis YxdJ response regulator as the inducer of expression for the cognate ABC transporter YxdLM
Open Access
- 1 August 2004
- journal article
- Published by Microbiology Society in Microbiology
- Vol. 150 (8), 2609-2617
- https://doi.org/10.1099/mic.0.27155-0
Abstract
The genome ofBacillus subtilis, like those of some other AT-rich Gram-positive bacteria, has the uncommon feature of containing several copies of arrangements in which the genes encoding two-component and cognate ABC transporter systems are adjacent. As the function of one of these systems, the product of theyxdlocus, is still unknown, it was analysed further in order to get some clues on the physiological role of the gene products it encodes. TheyxdJgene was shown to encode a DNA-binding protein that directly controls transcription of the neighbouring operon encoding the ABC transporter YxdLM. Primer extension and DNase protection experiments allowed precise definition of theyxdLMtranscription start and controlling region. Two putative direct repeats were identified that are proposed to be the YxdJ response regulator binding sites. Whole-cell transcriptome analyses revealed that the YxdJ regulon is extremely restricted. In addition to theyxdJKLMyxeAoperon, only a few genes involved in modifications of the bacterial cell wall were shown to be regulated by YxdJ.Keywords
This publication has 28 references indexed in Scilit:
- YtsCD and YwoA, two independent systems that confer bacitracin resistance toBacillus subtilisFEMS Microbiology Letters, 2003
- Rapid orientated cloning in a shuttle vector allowing modulated gene expression inBacillus subtilisFEMS Microbiology Letters, 2001
- Sigma A recognition sites in the Bacillus subtilis genomeMicrobiology, 2001
- The essential two-component regulatory system encoded by yycF and yycG modulates expression of the ftsAZ operon in Bacillus subtilisMicrobiology, 2000
- Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoterJournal of Bacteriology, 1995
- Compilation and analysus ofBacillus SubtilisσA-dependent promoter sequences: evidence for extended contact between RNA polymerse and upstream promoter DNANucleic Acids Research, 1995
- Sequential action of two-component genetic switches regulates the PHO regulon in Bacillus subtilisJournal of Bacteriology, 1994
- Anaerobic control of colicin E1 productionJournal of Bacteriology, 1992
- A family of related ATP-binding subunits coupled to many distinct biological processes in bacteriaNature, 1986
- BACTERIAL PERIPLASMIC TRANSPORT SYSTEMS: STRUCTURE, MECHANISM, AND EVOLUTIONAnnual Review of Biochemistry, 1986