Calreticulin mutants as oncogenic rogue chaperones for TpoR and traffic-defective pathogenic TpoR mutants
- 20 June 2019
- journal article
- research article
- Published by American Society of Hematology in Blood
- Vol. 133 (25), 2669-2681
- https://doi.org/10.1182/blood-2018-09-874578
Abstract
Calreticulin (CALR) +1 frameshift mutations in exon 9 are prevalent in myeloproliferative neoplasms. Mutant CALRs possess a new C-terminal sequence rich in positively charged amino acids, leading to activation of the thrombopoietin receptor (TpoR/MPL). We show that the new sequence endows the mutant CALR with rogue chaperone activity, stabilizing a dimeric state and transporting TpoR and mutants thereof to the cell-surface in states that would not pass quality control, and this function is absolutely required for oncogenic transformation. Mutant CALRs determine traffic via the secretory pathway of partially immature TpoR, as they protect N117-linked glycans from further processing in the Golgi. A number of engineered or disease-associated TpoRs, such as TpoR/MPL R102P, which causes congenital thrombocytopenia are rescued for traffic and function by mutant CALRs, which can also overcome endoplasmic reticulum retention signals on TpoR. Besides requiring N-glycosylation of TpoR, mutant CALRs require a hydrophobic patch located in the extracellular domain of TpoR to induce TpoR thermal stability and initial intracellular activation, while full activation requires cell surface localization of TpoR. Thus, mutant CALRs are rogue chaperones for TpoR and traffic-defective TpoR mutants, a function required for the oncogenic effects. Key points CALR mutants rescue cell surface localization of traffic deficient TpoR including R102P that causes congenital thrombocytopenia. Oncogenic TpoR activation by CALR mutants requires interaction, stabilization and cell surface localization of the TpoR-CALR complex.Keywords
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