A Biomimetic Receptor System for Sialic Acid Based on Molecular Imprinting
- 1 January 1996
- journal article
- research article
- Published by Informa UK Limited in Analytical Letters
- Vol. 29 (2), 157-170
- https://doi.org/10.1080/00032719608000997
Abstract
A new type of a fluorescent receptor system specific for sialic acid has been prepared. For the preparation of this artificial receptor a polymerization reaction using allylamine, vinylphenylboronic acid and ethylene glycol dimethacrylate in the presence of sialic acid as template was carried out. After splitting off the template molecules, a suspension of the polymer was used in reaction with OPA reagents, both in the presence and absence of sugars. It was found, that sialic acid promoted the fluorescence development in this system, probably as a result of the so-called “gate effect”, resembling natural occurring receptors. Rapid detection of sialic acid in the concentration range of 0.5–10 μM was shown possible within 40 minutes. Our method seems applicable for the development of optical sensors; combining the high sensitivity and selectivity of biological receptors and high stability of synthetic polymeric materials.Keywords
This publication has 16 references indexed in Scilit:
- Dansyl-β-cyclodextrins as Fluorescent Sensors Responsive to Organic CompoundsBulletin of the Chemical Society of Japan, 1994
- Nondestructive Detection of Acetylcholine in Protic Media: Artificial-Signaling Acetylcholine ReceptorsJournal of the American Chemical Society, 1994
- Molecular Imprints Make a MarkScience, 1994
- Molecular imprinting of flat polycondensed aromatic molecules in macroporous polymersPolymer, 1993
- Fluorescent Chemosensors for Ion and Molecule RecognitionACS Symposium Series, 1993
- Some studies of molecularly-imprinted polymer membranes in combination with field-effect devicesSensors and Actuators A: Physical, 1993
- Influence of polymer morphology on the ability of imprinted network polymers to resolve enantiomersJournal of Chromatography A, 1993
- Drug assay using antibody mimics made by molecular imprintingNature, 1993
- Enzyme-analog-built polymers. 27. Racemic resolution of free sugars with macroporous polymers prepared by molecular imprinting. Selectivity dependence on the arrangement of functional groups versus spatial requirementsThe Journal of Organic Chemistry, 1991
- Substrate-selective polymeric membranes. Selective transfer of nucleic acids componentsBiopolymers and Cell, 1990