Functional differentiation of a clone resembling embryonic cortical interneuron progenitors
- 23 September 2008
- journal article
- research article
- Published by Wiley in Developmental Neurobiology
- Vol. 68 (14), 1549-1564
- https://doi.org/10.1002/dneu.20679
Abstract
We have generated clones (L2.3 and RG3.6) of neural progenitors with radial glial properties from rat E14.5 cortex that differentiate into astrocytes, neurons, and oligodendrocytes. Here, we describe a different clone (L2.2) that gives rise exclusively to neurons, but not to glia. Neuronal differentiation of L2.2 cells was inhibited by bone morphogenic protein 2 (BMP2) and enhanced by Sonic Hedgehog (SHH) similar to cortical interneuron progenitors. Compared with L2.3, differentiating L2.2 cells expressed significantly higher levels of mRNAs for glutamate decarboxylases (GADs ), DLX transcription factors, calretinin , calbindin , neuropeptide Y (NPY ), and somatostatin . Increased levels of DLX‐2 , GADs , and calretinin proteins were confirmed upon differentiation. L2.2 cells differentiated into neurons that fired action potentials in vitro , and their electrophysiological differentiation was accelerated and more complete when cocultured with developing astroglial cells but not with conditioned medium from these cells. The combined results suggest that clone L2.2 resembles GABAergic interneuron progenitors in the developing forebrain. © 2008 Wiley Periodicals, Inc. Develop Neurobiol 2008Keywords
This publication has 86 references indexed in Scilit:
- The Cortical Ancestry of Oligodendrocytes: Common Principles and Novel FeaturesDevelopmental Neuroscience, 2003
- Microtubules are critical for radial glial morphology: Possible regulation by MAPs and MARKsGlia, 2003
- Distinct cortical migrations from the medial and lateral ganglionic eminencesDevelopment, 2001
- Pluripotent Stem Cells Engrafted into the Normal or Lesioned Adult Rat Spinal Cord Are Restricted to a Glial LineageExperimental Neurology, 2001
- Characterization of CNS Precursor Subtypes and Radial GliaDevelopmental Biology, 2001
- Isolation of radial glial cells by fluorescent-activated cell sorting reveals a neuronal lineageDevelopment, 2000
- Cell Migration from the Ganglionic Eminences Is Required for the Development of Hippocampal GABAergic InterneuronsNeuron, 2000
- Oligodendrocyte Precursor Cells Reprogrammed to Become Multipotential CNS Stem CellsScience, 2000
- Origin and Molecular Specification of Striatal InterneuronsJournal of Neuroscience, 2000
- Establishment and Properties of a Growth Factor-Dependent, Perpetual Neural Stem Cell Line from the Human CNSExperimental Neurology, 2000