Quantification of Gold Nanoparticle Cell Uptake Under Controlled Biological Conditions and Adequate Resolution
- 1 April 2014
- journal article
- research article
- Published by Taylor & Francis Ltd in Nanomedicine
- Vol. 9 (5), 607-621
- https://doi.org/10.2217/nnm.13.24
Abstract
Aim: We examined cellular uptake mechanisms of fluorescently labeled polymer-coated gold nanoparticles (NPs) under different biological conditions by two quantitative, microscopic approaches. Materials & methods: Uptake mechanisms were evaluated using endocytotic inhibitors that were tested for specificity and cytotoxicity. Cellular uptake of gold NPs was analyzed either by laser scanning microscopy or transmission electron microscopy, and quantified by means of stereology using cells from the same experiment. Results: Optimal inhibitor conditions were only achieved with chlorpromazine (clathrin-mediated endocytosis) and methyl-β-cyclodextrin (caveolin-mediated endocytosis). A significant methyl-β-cyclodextrin-mediated inhibition (63–69%) and chlorpromazine-mediated increase (43–98%) of intracellular NPs was demonstrated with both imaging techniques, suggesting a predominant uptake via caveolin-medicated endocytois. Transmission electron microscopy imaging revealed more than 95% of NPs localized in intrac...This publication has 69 references indexed in Scilit:
- Nanoparticles and Cells: An Interdisciplinary ApproachChimia, 2012
- Effects of Transport Inhibitors on the Cellular Uptake of Carboxylated Polystyrene Nanoparticles in Different Cell LinesPLOS ONE, 2011
- Uptake of nanoparticles by alveolar macrophages is triggered by surfactant protein ANanomedicine: Nanotechnology, Biology and Medicine, 2011
- Intracellular imaging of nanoparticles: Is it an elemental mistake to believe what you see?Particle and Fibre Toxicology, 2010
- Size and Dynamics of Caveolae Studied Using Nanoparticles in Living Endothelial CellsACS Nano, 2009
- A Raft-derived, Pak1-regulated Entry Participates in α2β1 Integrin-dependent Sorting to CaveosomesMolecular Biology of the Cell, 2008
- Mechanistic Studies of a Peptidic GRP78 Ligand for Cancer Cell-Specific Drug DeliveryMolecular Pharmaceutics, 2007
- Targeting of nanoparticles to the clathrin-mediated endocytic pathwayBiochemical and Biophysical Research Communications, 2007
- Visualization and quantitative analysis of nanoparticles in the respiratory tract by transmission electron microscopyParticle and Fibre Toxicology, 2007
- Freeze-fracture evidence for the presence of cholesterol in particle-free patches of basal disks and the plasma membrane of retinal rod outer segments of mice and frogs.The Journal of cell biology, 1979