The Putative Coupling Protein TcpA Interacts with Other pCW3-Encoded Proteins To Form an Essential Part of the Conjugation Complex
- 1 May 2009
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 191 (9), 2926-2933
- https://doi.org/10.1128/jb.00032-09
Abstract
Conjugative plasmids encode antibiotic resistance determinants or toxin genes in the anaerobic pathogen Clostridium perfringens. The paradigm conjugative plasmid in this bacterium is pCW3, a 47-kb tetracycline resistance plasmid that encodes the unique tcp transfer locus. The tcp locus consists of 11 genes, intP and tcpA-tcpJ, at least three of which, tcpA, tcpF, and tcpH, are essential for the conjugative transfer of pCW3. In this study we examined protein-protein interactions involving TcpA, the putative coupling protein. Use of a bacterial two-hybrid system identified interactions between TcpA and TcpC, TcpG, and TcpH. This analysis also demonstrated TcpA, TcpC, and TcpG self-interactions, which were confirmed by chemical cross-linking studies. Examination of a series of deletion and site-directed derivatives of TcpA identified the domains and motifs required for these interactions. Based on these results, we have constructed a model for this unique conjugative transfer apparatus.Keywords
This publication has 38 references indexed in Scilit:
- Functional Characterization and Localization of the TcpH Conjugation Protein fromClostridium perfringensJournal of Bacteriology, 2008
- TcpA, an FtsK/SpoIIIE Homolog, Is Essential for Transfer of the Conjugative Plasmid pCW3 inClostridium perfringensJournal of Bacteriology, 2007
- Epsilon-Toxin Plasmids of Clostridium perfringens Type D Are ConjugativeJournal of Bacteriology, 2007
- In Vivo Oligomerization of the F Conjugative Coupling Protein TraDJournal of Bacteriology, 2007
- A Type IV-Secretion-Like System Is Required for Conjugative DNA Transport of Broad-Host-Range Plasmid pIP501 in Gram-Positive BacteriaJournal of Bacteriology, 2007
- Functional Identification of Conjugation and Replication Regions of the Tetracycline Resistance Plasmid pCW3 from Clostridium perfringensJournal of Bacteriology, 2006
- F factor conjugation is a true type IV secretion systemFEMS Microbiology Letters, 2003
- VirB11 ATPases are dynamic hexameric assemblies: new insights into bacterial type IV secretionThe EMBO Journal, 2003
- Motors and switches: AAA+ machines within the replisomeNature Reviews Molecular Cell Biology, 2002
- The structure of the E. coli recA protein monomer and polymerNature, 1992