Conventional multiple or microdissection testicular sperm extraction: a comparative study
Open Access
- 1 November 2002
- journal article
- research article
- Published by Oxford University Press (OUP) in Human Reproduction
- Vol. 17 (11), 2924-2929
- https://doi.org/10.1093/humrep/17.11.2924
Abstract
BACKGROUND: Testicular sperm extraction (TESE) with ICSI is becoming the first-line treatment for non-obstructive azoospermia (NOA). Recently, the sperm retrieval rate (SRR) by microdissection TESE was reported to be higher than by conventional TESE. However, a comprehensive comparison between multiple and microdissection TESE patients including histological findings has not been reported. METHODS: Patients with NOA who underwent microdissection TESE (n = 56) or multiple TESE (n = 37) were compared. Pre-operative characteristics were similar between groups. In addition, microscopic findings during microdissection TESE also were investigated. RESULTS: Operative time was significantly longer for microdissection TESE than for multiple TESE. Histological examination suggested that spermatogenesis was relatively more impaired in the microdissection TESE group than in the multiple TESE group. Despite this, SRR by microdissection TESE (42.9%) appeared higher than by conventional TESE (35.1%) although this observation failed to reach statistical significance. Seventeen of 26 patients (65.4%) with heterogeneous tubule were successful for sperm retrieval. No severe operative complications occurred in any patient in either group, and no patient required post-operative hormone replacement to treat hypogonadism. CONCLUSIONS: Microsurgical technique is safe and may improve SRR for TESE in a variety of patients with NOA, especially patients with heterogeneous testicular tubules.Keywords
This publication has 25 references indexed in Scilit:
- A prospective study of multiple needle biopsies versus a single open biopsy for testicular sperm extraction in men with non-obstructive azoospermiaHuman Reproduction, 1998
- Multiple testicular sampling in non-obstructive azoospermia--is it necessary?Human Reproduction, 1998
- Microdissection TESE: sperm retrieval in non-obstructive azoopsermia. VIDEOHuman Reproduction Update, 1998
- Are there any predictive factors for successful testicular sperm recovery in azoospermic patients?Human Reproduction, 1997
- Ooplasmic round spermatid nuclear injection procedures as an experimental treatment for nonobstructive azoospermiaJournal of Assisted Reproduction and Genetics, 1997
- Viable Embryos from Injection of Round Spermatids into OocytesThe New England Journal of Medicine, 1995
- Pregnancies after testicular sperm extraction and intracytoplasmic sperm injection in non-obstructive azoospermiaHuman Reproduction, 1995
- High fertilization and pregnancy rate after intracytoplasmic sperm injection with spermatozoa obtained from testicle biopsyHuman Reproduction, 1995
- Surveillance case definition for AIDS in resource-poor countriesThe Lancet, 1993
- Testicular Biopsy Score Count – A Method for Registration of Spermatogenesis in Human Testes: Normal Values and Results in 335 Hypogonadal MalesHormone Research in Paediatrics, 1970