Curcumin Inhibits LPS-Induced CCL2 Expression via JNK Pathway in C6 Rat Astrocytoma Cells
- 13 March 2012
- journal article
- Published by Springer Science and Business Media LLC in Cellular and Molecular Neurobiology
- Vol. 32 (6), 1003-1010
- https://doi.org/10.1007/s10571-012-9816-4
Abstract
The important role of neuroinflammation in many chronic and acute pathological conditions of the central nervous system is widely recognized. Curcumin is a major component of turmeric and reportedly has anti-inflammatory and anti-oxidant effects. This study investigated the inhibitory effect of curcumin on lipopolysacharide (LPS)-induced chemokine CCL2 (or monocyte chemoattractant protein-1, MCP-1) production and whether the effect is mediated by mitogen-activated protein kinases (MAPKs) in the rat astrocytoma cell C6. We observed that LPS (1 μg/ml) induced the upregulation of CCL2 mRNA and protein in C6. Treatment with curcumin (2.5, 10, and 25 μM) decreased the expression of CCL2 mRNA and protein in a dose-dependent manner under treatment with LPS. Additionally, the c-jun N-terminal kinase (JNK) inhibitor (SP600125) dose-dependently inhibited LPS-induced CCL2 upregulation, whereas the MAPK kinase (MEK) inhibitor (PD98059) only had a mild effect and the p38 MAPK inhibitor (SB203580) had no effect. Finally, western blot showed that LPS induced rapid JNK activation and curcumin reduced LPS-induced phosphoJNK (pJNK) expression at 30 min after LPS stimulation. These data suggest that the anti-neuroinflammatory effect of curcumin relates to the downregulation of CCL2 expression through the JNK pathway in astrocytoma cells, which indicates a possible benefit from the use of curcumin in the treatment of neuroinflammation-associated disorders.Keywords
This publication has 54 references indexed in Scilit:
- Targeting Astrocyte Signaling for Chronic PainNeurotherapeutics, 2010
- Chemokines, neuronal–glial interactions, and central processing of neuropathic painPharmacology & Therapeutics, 2010
- MAP kinase and painBrain Research Reviews, 2009
- Chemokines as pain mediators and modulatorsCurrent Opinion In Anesthesiology, 2008
- Curcumin Structure-Function, Bioavailability, and Efficacy in Models of Neuroinflammation and Alzheimer's DiseaseJournal of Pharmacology and Experimental Therapeutics, 2008
- “Listening” and “talking” to neurons: Implications of immune activation for pain control and increasing the efficacy of opioidsBrain Research Reviews, 2007
- The role of inflammation in CNS injury and diseaseBritish Journal of Pharmacology, 2006
- Constitutive neuronal expression of CCR2 chemokine receptor and its colocalization with neurotransmitters in normal rat brain: Functional effect of MCP‐1/CCL2 on calcium mobilization in primary cultured neuronsJournal of Comparative Neurology, 2005
- Cytokine, chemokine and growth factor gene profiling of cultured human astrocytes after exposure to proinflammatory stimuliGlia, 2003
- Expression of chemokines and their receptors in human and simian astrocytes: Evidence for a central role of TNFα and IFNγ in CXCR4 and CCR5 modulationGlia, 2003