New Lignan Glycosides with Potent Antiinflammatory Effect, Isolated from Justicia ciliata

Abstract

Two new lignan glycosides, 4-O-[α-l-arabinopyranosyl-(1‘ ‘‘→2‘ ‘)-β-d-xylopyranosyl-(1‘ ‘‘ ‘→5‘ ‘)-β-d-apiofuranosyl]diphyllin (1), named ciliatoside A (1), and 4-O-{[β-d-apiofuranosyl-(1‘ ‘‘ ‘‘→3‘ ‘‘)-α-l-arabinopyranosyl-(1‘ ‘‘→2‘ ‘)][β-d-xylopyranosyl-(1‘ ‘‘ ‘→5‘ ‘)]-β-d-apiofuranosyl}diphyllin (2), named ciliatoside B (2), were isolated from the whole plant of Justicia ciliata. The structures of 1 and 2 were determined by spectral and chemical methods. Compounds 1 and 2 strongly inhibited the accumulation of NO₂^- in lipopolysaccharide-stimulated RAW 264.7 cells in a concentration-dependent manner with IC₅₀ values of 27.1 ± 1.6 and 29.4 ± 1.4 μM, respectively.