Time course changes of vasopressin-induced enlargement of the rat intrastrial space and the effects of a vasopressin type 2 antagonist

Abstract

Conclusion: The intrastrial space was enlarged remarkably at 20 min after vasopressin (VP) injection, and this enlargement of the intrastrial space was reduced by administration of OPC-31260 before VP injection. These results suggest that VP increases the influx of water from the perlymph to the basal cells via aquaporin (AQP) 2 and causes the formation of endolymphatic hydrops. Objectives: To investigate a time course of changes of the stria vascularis after VP injection and the influence of OPC-31260 on experimentally induced enlargement of the intrastrial space caused by VP injection. Materials and methods: In the time course study, Wistar rats were injected with 50 µg/kg of VP subcutaneously. The stria vascularis specimens were harvested at 10, 20, 30, and 60 min after VP injection. For OPC-31260 administration, animals were administered 100 mg/kg of OPC-31260 orally 1 h before receiving 50 µg/kg of VP subcutaneously. The specimens were harvested 20 min after VP injection. These specimens were observed using transmission electron microscopy. Results: In the time course study, the incidence of intrastrial space enlargement was 50%, 100%, 25%, and 0% for 10, 20, 30, and 60 min, respectively. In the study with OPC-31260 administration, the stria vascularis showed no morphological changes.