Inactivation of enzymes within spores ofBacillus megateriumATCC 19213 by hydroperoxides
- 1 May 1998
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 44 (5), 465-470
- https://doi.org/10.1139/w98-026
Abstract
The organic hydroperoxides t-butyl hydroperoxide, cumene hydroperoxide, and peracetic acid were found to act similarly to hydrogen peroxide in causing inactivation of enzymes within intact spores of Bacillus megaterium ATCC 19213 concomitant with mortality. Spores treated with lethal levels of the agents were germinated and permeabilized for enzyme assays. The hierarchy of sensitivities among enolase, glucose-6-phosphate dehydrogenase (G6Pdh), and pyruvate kinase to inactivation varied somewhat with the specific hydroperoxide used, possibly because of differences in the types of radicals generated. However, each agent inactivated each of the enzymes, albeit at different rates. Comparative assessments of enzyme inactivation by lethal levels of H2O2or by moist heat showed that some enzymes, such as G6Pdh, are highly sensitive to inactivation, while others, such as ATPases, are much more resistant. The enzymes G6Pdh and aldolase were highly sensitive to hydroperoxide inactivation and also to moist heat, while pyruvate kinase was much more sensitive to hydroperoxides than to moist heat. Our overall interpretation of the findings is that hydroperoxides and moist heat can produce cumulative damage to sensitive enzymes within spores, which progressively diminishes the capacities of the cells to undergo the outgrowth required for return to vegetative life.Key words: bacterial spores, hydroperoxides, Bacillus megaterium, enzyme inactivation.Keywords
This publication has 10 references indexed in Scilit:
- Killing bacterial spores by organic hydroperoxidesJournal of Industrial Microbiology & Biotechnology, 1997
- Hydroperoxide inactivation of enzymes within spores ofBacillus megateriumATCC19213FEMS Microbiology Letters, 1996
- Identification and characterization of a gene cluster involved in manganese oxidation by spores of the marine Bacillus sp. strain SG-1Journal of Bacteriology, 1996
- Oxidative Stress Response in Yeast: Purification and Some Properties of Oxidative Stress-inducible Glucose-6-phosphate Dehydrogenase fromHansenula mrakiiBioscience, Biotechnology, and Biochemistry, 1996
- Quasi-irreversible inhibition of enolase ofStreptococcus mutansby flourideFEMS Microbiology Letters, 1994
- The Bactericidal Action of Peroxides; An E.P.R. Spin-Trapping StudyFree Radical Research, 1994
- Bactericidal activity of alkyl peroxyl radicals generated by heme-iron-catalyzed decomposition of organic peroxidesArchives of Biochemistry and Biophysics, 1992
- Generation of oxy radicals in biosystemsMutation Research, 1989
- INFLUENCE OF MEDIA AND MEDIA CONSTITUENTS ON RECOVERY OF BACTERIAL SPORES EXPOSED TO HYDROGEN PEROXIDEJournal of Food Science, 1979
- ALTERATIONS IN METAL CONTENT OF SPORES OFBACILLUS MEGATERIUMAND THE EFFECT ON SOME SPORE PROPERTIESJournal of Bacteriology, 1959