AN AUTORADIOGRAPHIC STUDY OF THE3H-URIDINE AND3H-THYMIDINE INCORPORATION IN THE REGENERATING MOUSE LIVER
- 1 September 1977
- journal article
- Published by Wiley in Cell Proliferation
- Vol. 10 (5), 477-485
- https://doi.org/10.1111/j.1365-2184.1977.tb00866.x
Abstract
An autoradiographic study was made of the 3H-uridine incorporation into RNA and DNA in nucleus and cytoplasm of parenchymal cells in the regenerating liver of the mouse after a pulse time of 2 hr. After a decreased uptake of precursor into the parenchymal nucleus during the first 6 hr compared with the normal value, incorporation increased and was maximal at 36 hr; normal values were restored at 72 hr. The cytoplasmic labelling, after an initial small decrease, reached a maximum at 12 hr; this changed to normal 48 hr after hepatectomy. RNase-digestion of the liver sections left a small incorporation in both nucleus and cytoplasm: presumably DNA. This incorporation is maximal at 12 hr over the nucleus and at 24 hr over the cytoplasm. After a 2 hr pulse of 3H-thymidine, there was a marked uptake of the precursor into DNA about 24 hr after hepatectomy. This was maximal at 48 hr and reached normal values at 72 hr. A small amount of incorporation of 3H-thymidine into DNA was seen immediately after the operation, and this population of weakly labelled nuclei was still rather large 72 hr later.Keywords
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