Berberine Inhibited the Growth of Thyroid Cancer Cell Lines 8505C and TPC1
Open Access
- 1 January 2012
- journal article
- Published by Yonsei University College of Medicine in Yonsei Medical Journal
- Vol. 53 (2), 346-351
- https://doi.org/10.3349/ymj.2012.53.2.346
Abstract
Thyroid cancer is the most common malignancy in Korean females and can be treated with good prognosis. However, drugs to treat aggressive types of thyroid cancer such as poorly differentiated or anaplastic thyroid cancer have not yet been established. To that end, we analyzed the effects of berberine on human thyroid cancer cell lines to determine whether this compound is useful in the treatment of aggressive thyroid cancer. The two thyroid cancer cell lines 8505C and TPC1, under adherent culture conditions, were treated with berberine and analyzed for changes in cell growth, cell cycle duration, and degree of apoptosis. Following berberine treatment, both cell lines showed a dose-dependent reduction in growth rate. 8505C cells showed significantly increased levels of apoptosis following berberine treatment, whereas TPC1 cells showed cell cycle arrest at the G0/G1 phase. Immunobloting of p-27 expression following berberine treatment showed that berberine induced a little up-regulation of p-27 in 8505c cells but relatively high up-regulation of p-27 in TPC1 cells. These results suggest that berberine treatment of thyroid cancer can inhibit proliferation through apoptosis and/or cell cycle arrest. Thus, berberine may be a novel anticancer drug for the treatment of poorly differentiated or anaplastic thyroid cancer.Keywords
This publication has 24 references indexed in Scilit:
- Berberine modulates AP-1 activity to suppress HPV transcription and downstream signaling to induce growth arrest and apoptosis in cervical cancer cellsMolecular Cancer, 2011
- Berberine induces dendritic cell apoptosis and has therapeutic potential for rheumatoid arthritisArthritis & Rheumatism, 2010
- Proliferation and survival molecules implicated in the inhibition of BRAF pathway in thyroid cancer cells harbouring different genetic mutationsBMC Cancer, 2009
- Nationwide Cancer Incidence in Korea, 2003-2005Cancer Research and Treatment, 2009
- p53 cooperates berberine‐induced growth inhibition and apoptosis of non‐small cell human lung cancer cells in vitro and tumor xenograft growth in vivoMolecular Carcinogenesis, 2008
- Cancer Statistics, 2007CA: A Cancer Journal for Clinicians, 2007
- Mutant B-RAF signaling and cyclin D1 regulate Cks1/S-phase kinase-associated protein 2-mediated degradation of p27Kip1 in human melanoma cellsOncogene, 2006
- Inhibitory effect of berberine on the invasion of human lung cancer cells via decreased productions of urokinase-plasminogen activator and matrix metalloproteinase-2Toxicology and Applied Pharmacology, 2006
- Effect of berberine on proliferation, biosynthesis of macromolecules, cell cycle and induction of intercalation with DNA, dsDNA damage and apoptosis in Ehrlich ascites carcinoma cellsJournal of Pharmacy and Pharmacology, 2006
- Effect of berberine on proliferation, cell cycle and apoptosis in HeLa and L1210 cellsJournal of Pharmacy and Pharmacology, 2003