Applications of CpG Island Microarrays for High-Throughput Analysis of DNA Methylation
Open Access
- 1 August 2002
- journal article
- research article
- Published by Elsevier BV in Journal of Nutrition
- Vol. 132 (8), 2430S-2434S
- https://doi.org/10.1093/jn/132.8.2430s
Abstract
Differential methylation hybridization (DMH) is a high-throughput microarray technique designed to identify changes in DNA methylation patterns commonly observed in cancer and other disease states. The DMH methodology comprises three fundamental components: the arraying of CpG island clones on glass slides, the preparation of the sample amplicons under investigation, and the hybridization of amplicon targets onto the CpG island microarray. Herein, we outline the DMH protocol and illustrate its utility and the validation approaches used in analyzing the hypermethylation profile of breast tumor and normal samples.Keywords
This publication has 18 references indexed in Scilit:
- The role of folic acid and Vitamin B12 in genomic stability of human cellsMutation Research, 2001
- Transcription of IAP endogenous retroviruses is constrained by cytosine methylationNature Genetics, 1998
- DNA methylation and imprinting: why bother?Trends in Genetics, 1997
- Role for DNA methylation in genomic imprintingNature, 1993
- Folate, Methionine, and Alcohol Intake and Risk of Colorectal AdenomaJNCI Journal of the National Cancer Institute, 1993
- Pernicious anemia and subsequent cancer. A population-based cohort studyCancer, 1993
- X chromosome inactivation and DNA methylationPublished by Springer Science and Business Media LLC ,1993
- The essentials of DNA methylationCell, 1992
- Improvement in Bronchial Squamous Metaplasia in Smokers Treated With Folate and Vitamin B12Published by American Medical Association (AMA) ,1988
- Improvement in cervical dysplasia associated with folic acid therapy in users of oral contraceptivesThe American Journal of Clinical Nutrition, 1982