Chylomicrons Promote Intestinal Absorption and Systemic Dissemination of Dietary Antigen (Ovalbumin) in Mice
Open Access
- 24 December 2009
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 4 (12), e8442
- https://doi.org/10.1371/journal.pone.0008442
Abstract
A small fraction of dietary protein survives enzymatic degradation and is absorbed in potentially antigenic form. This can trigger inflammatory responses in patients with celiac disease or food allergies, but typically induces systemic immunological tolerance (oral tolerance). At present it is not clear how dietary antigens are absorbed. Most food staples, including those with common antigens such as peanuts, eggs, and milk, contain long-chain triglycerides (LCT), which stimulate mesenteric lymph flux and postprandial transport of chylomicrons through mesenteric lymph nodes (MLN) and blood. Most dietary antigens, like ovalbumin (OVA), are emulsifiers, predicting affinity for chylomicrons. We hypothesized that chylomicron formation promotes intestinal absorption and systemic dissemination of dietary antigens. Absorption of OVA into MLN and blood was significantly enhanced when OVA was gavaged into fasted mice together with LCT compared with medium-chain triglycerides (MCT), which do not stimulate chylomicron formation. The effect of LCT was blocked by the addition of an inhibitor of chylomicron secretion, Pluronic L-81. Adoptively transferred OVA-specific DO11.10 T-cells proliferated more extensively in peripheral lymph nodes when OVA was gavaged with LCT than with MCT or LCT plus Pluronic L-81, suggesting that dietary OVA is systemically disseminated. Most dietary OVA in plasma was associated with chylomicrons, suggesting that these particles mediate systemic antigen dissemination. Intestinal-epithelial CaCo-2 cells secreted more cell-associated, exogenous OVA when stimulated with oleic-acid than with butyric acid, and the secreted OVA appeared to be associated with chylomicrons. Postprandial chylomicron formation profoundly affects absorption and systemic dissemination of dietary antigens. The fat content of a meal may affect immune responses to dietary antigens by modulating antigen absorption and transport.Keywords
This publication has 40 references indexed in Scilit:
- Intestinal lipid absorptionAmerican Journal of Physiology-Endocrinology and Metabolism, 2009
- Retinoic acid inhibits Th17 polarization and enhances FoxP3 expression through a Stat-3/Stat-5 independent signaling pathwayBlood, 2008
- Small intestine lamina propria dendritic cells promote de novo generation of Foxp3 T reg cells via retinoic acidThe Journal of Experimental Medicine, 2007
- Shotgun proteomics implicates protease inhibition and complement activation in the antiinflammatory properties of HDLJCI Insight, 2007
- Diverse range of small peptides associated with high-density lipoproteinBiochemical and Biophysical Research Communications, 2006
- Quantification of Postprandial Triglyceride-Rich Lipoproteins in Healthy Men by Retinyl Ester Labeling and Simultaneous Measurement of Apolipoproteins B-48 and B-100Arteriosclerosis, Thrombosis, and Vascular Biology, 1995
- Chylomicrons alter the fate of endotoxin, decreasing tumor necrosis factor release and preventing death.JCI Insight, 1993
- Maintenance of the macromolecular barrier at cell extrusion sites in intestinal epithelium: Physiological rearrangement of tight junctionsThe Journal of Membrane Biology, 1990
- Adsorption onto an oil surface and emulsifying properties of bovine .alpha.s1-casein in relation to its molecular structureJournal of Agricultural and Food Chemistry, 1983
- The metabolism of very low density lipoprotein proteins I. Preliminary in vitro and in vivo observationsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1972