Butyric Acid- and Dimethyl Disulfide-Assimilating Microorganisms in a Biofilter Treating Air Emissions from a Livestock Facility
- 15 December 2011
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 77 (24), 8595-8604
- https://doi.org/10.1128/aem.06175-11
Abstract
Biofiltration has proven an efficient tool for the elimination of volatile organic compounds (VOCs) and ammonia from livestock facilities, thereby reducing nuisance odors and ammonia emissions to the local environment. The active microbial communities comprising these filter biofilms have not been well characterized. In this study, a trickle biofilter treating air from a pig facility was investigated and proved efficient in removing carboxylic acids (>70% reduction), mainly attributed to the primary filter section within which reduced organic sulfur compounds were also depleted (up to 50%). The secondary filter eliminated several aromatic compounds: phenol (81%), p -cresol (89%), 4-ethylphenol (68%), indole (48%), and skatole (69%). The active butyric acid degrading bacterial community of an air filter sample was identified by DNA stable-isotope probing (DNA-SIP) and microautoradiography, combined with fluorescence in situ hybridization (MAR-FISH). The predominant 16S rRNA gene sequences from a clone library derived from “heavy” DNA from [ 13 C 4 ]butyric acid incubations were Microbacterium , Gordonia , Dietzia , Rhodococcus , Propionibacterium , and Janibacter , all from the Actinobacteria. Actinobacteria were confirmed and quantified by MAR-FISH as being the major bacterial phylum assimilating butyric acid along with several Burkholderiales -related Betaproteobacteria . The active bacterial community assimilating dimethyl disulfide (DMDS) was characterized by DNA-SIP and MAR-FISH and found to be associated with the Actinobacteria , along with a few representatives of Flavobacteria and Sphingobacteria . Interestingly, ammonia-oxidizing Betaproteobacteria were also implicated in DMDS degradation, as were fungi. Thus, multiple isotope-based methods provided complementary data, enabling high-resolution identification and quantitative assessments of odor-eliminating Actinobacteria -dominated populations of these biofilter environments.Keywords
This publication has 45 references indexed in Scilit:
- Nucleic acid contamination of glycogen used in nucleic acid precipitation and assessment of linear polyacrylamide as an alternative co-precipitantBioTechniques, 2009
- Hydrocarbon degradation by Dietzia sp. A14101 isolated from an oil reservoir model columnAntonie van Leeuwenhoek, 2009
- Distribution and Rate of Microbial Processes in an Ammonia-Loaded Air Filter BiofilmApplied and Environmental Microbiology, 2009
- SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARBNucleic Acids Research, 2007
- Pathways in the Degradation of Hydrolyzed Alcohols of Butyl Benzyl Phthalate in Metabolically Diverse Gordonia sp.Strain MTCC 4818Microbial Physiology, 2005
- Potentials of biological oxidation processes for the treatment of spent sulfidic caustics containing thiolsWater Research, 2004
- Investigation of Artefact Formation During Analysis of Volatile Sulphur Compounds Using Solid Phase Microextraction (SPME)Chromatographia, 2004
- Bellerophon: a program to detect chimeric sequences in multiple sequence alignmentsBioinformatics, 2004
- Stable-Isotope-Based Labeling of Styrene-Degrading Microorganisms in BiofiltersApplied and Environmental Microbiology, 2001
- 216. The solubility of non-electrolytes. Part II. The influence of the polar group on the free energy of hydration of aliphatic compoundsJournal of the Chemical Society, 1935