HPLC Trennung und quantitative Bestimmung der Ginsenoside von Panax ginseng, Panax quinquefolium und von Ginseng–Spezialitäten

Abstract

A new method for separation and quantitative determination of ginsenosides Rg₂, Rf, Rg₁ and Re in Panax ginseng, Panax quinquefolium and in pharmaceutical drug preparations with HPLC was elaborated. μPorasil column using n–heptane–n–butanol–aceto–nitril–water (1000:446:132:36) as mobile phase was employed. The ginsenosides were separated in 40 min. A reversed–phase–system with a μBondapak C₁₈ column using methanol–water (440:560) as eluent was suitable for the separation of ginsenoside Rg₁. The reversed–phase–system is adoptable for routine analysis of Ginseng extracts. The detection limit for Rg_t with the spectrophotometer LC–55 (Perkin–Elmer) was at 207 nm 300 ng at a signal–to–noice ratio of 10:1. The relative standard deviation is depending from the content of ginsenosides. For ginsenoside contents of 1 % it was circa 1 °/o.