Limitations of Direct Immunoassays for Measuring Circulating Estradiol Levels in Postmenopausal Women and Men in Epidemiologic Studies
Open Access
- 1 April 2010
- journal article
- Published by American Association for Cancer Research (AACR) in Cancer Epidemiology, Biomarkers & Prevention
- Vol. 19 (4), 903-906
- https://doi.org/10.1158/1055-9965.epi-10-0081
Abstract
Serum estradiol (E2) serves as an important diagnostic marker in a variety of clinical conditions. In epidemiologic studies, E2 is commonly used to define the etiologic role of estrogen in hormone-related cancers and chronic conditions. Having an accurate and reliable E2 assay is of critical importance in these studies, especially when measuring the very low E2 levels (2 antibodies, direct RIAs without purification steps lack specificity for E2 and can substantially overestimate E2 levels. Although direct E2 RIAs using commercial kits are simpler, less time consuming, and less expensive and require less sample volume than conventional RIAs with preceding purification steps, their lack of sensitivity and specificity makes them invalid for measuring circulating E2 levels in epidemiologic studies of postmenopausal women or men. Instead, we recommend the use of a well-validated RIA with purification steps to improve sensitivity and specificity and to help achieve the necessary accuracy and reliability needed for epidemiologic studies. Cancer Epidemiol Biomarkers Prev; 19(4); 903–6. ©2010 AACR.Keywords
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