Detection of endogenous biomolecules in Barrett's esophagus by Fourier transform infrared spectroscopy
- 2 October 2007
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 104 (40), 15864-15869
- https://doi.org/10.1073/pnas.0707567104
Abstract
Fourier transform infrared (FTIR) spectroscopy provides a unique molecular fingerprint of tissue from endogenous sources of light absorption; however, specific molecular components of the overall FTIR signature of precancer have not been characterized. In attenuated total reflectance mode, infrared light penetrates only a few microns of the tissue surface, and the influence of water on the spectra can be minimized, allowing for the analyses of the molecular composition of tissues. Here, spectra were collected from 98 excised specimens of the distal esophagus, including 38 squamous, 38 intestinal metaplasia (Barrett's), and 22 gastric, obtained endoscopically from 32 patients. We show that DNA, protein, glycogen, and glycoprotein comprise the principal sources of infrared absorption in the 950- to 1,800-cm(-1) regime. The concentrations of these biomolecules can be quantified by using a partial least-squares fit and used to classify disease states with high sensitivity, specificity, and accuracy. Moreover, use of FTIR to detect premalignant (dysplastic) mucosa results in a sensitivity, specificity, positive predictive value, and total accuracy of 92%, 80%, 92%, and 89%, respectively, and leads to a better interobserver agreement between two gastrointestinal pathologists for dysplasia (kappa = 0.72) versus histology alone (kappa = 0.52). Here, we demonstrate that the concentration of specific biomolecules can be determined from the FTIR spectra collected in attenuated total reflectance mode and can be used for predicting the underlying histopathology, which will contribute to the early detection and rapid staging of many diseases.Keywords
This publication has 32 references indexed in Scilit:
- Identification of infrared absorption spectral characteristics of synovial fluid of horses with osteochondrosis of the tarsocrural jointAmerican Journal of Veterinary Research, 2007
- Raman spectroscopy: elucidation of biochemical changes in carcinogenesis of oesophagusBritish Journal of Cancer, 2006
- FOURIER TRANSFORM INFRARED VIBRATIONAL SPECTROSCOPIC IMAGING: Integrating Microscopy and Molecular RecognitionAnnual Review of Physical Chemistry, 2005
- Infrared spectroscopic imaging for histopathologic recognitionNature Biotechnology, 2005
- Diagnosis of Gastric Inflammation and Malignancy in Endoscopic Biopsies Based on Fourier Transform Infrared SpectroscopyClinical Chemistry, 2005
- Near-infrared fiber optic spectroscopy as a novel diagnostic tool for the detection of pancreatic cancerJournal of Biomedical Optics, 2005
- Fourier transform infrared microspectroscopy as a quantitative diagnostic tool for assignment of premalignancy grading in cervical neoplasiaJournal of Biomedical Optics, 2004
- Infrared Spectroscopic Studies of Major Cellular Components. Part I: The Effect of Hydration on the Spectra of ProteinsApplied Spectroscopy, 2001
- FT-IR Internal Reflection Spectroscopy Using Regular Polygonal Internal Reflection ElementsApplied Spectroscopy, 1998
- Fourier Transform Infrared SpectroscopyScience, 1976