Identification of Hydrophobic Tags for the Degradation of Stabilized Proteins
- 23 January 2012
- journal article
- research article
- Published by Wiley in ChemBioChem
- Vol. 13 (4), 538-541
- https://doi.org/10.1002/cbic.201100793
Abstract
New HyTs are a knockout: We previously reported that labeling HaloTag proteins with low molecular weight hydrophobic tags (HyTs) leads to targeted degradation of HaloTag fusion proteins. In this report, we employed a chemical approach to extend this hydrophobic tagging methodology to highly stabilized proteins by synthesizing and evaluating a library of HyTs, which led to the identification of HyT36.Keywords
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