Oncogene Amplification in Squamous Cell Carcinoma of the Oral Cavity
Open Access
- 1 May 1989
- journal article
- Published by Wiley in Japanese Journal of Cancer Research
- Vol. 80 (5), 430-437
- https://doi.org/10.1111/j.1349-7006.1989.tb02332.x
Abstract
We have determined the prevalence of amplification of c‐myc, N‐myc, L‐myc, H‐ras, Ki‐ras, and N‐ras oncogenes in 23 cases of squamous cell carcinoma of the oral cavity, using Southern hybridization analysis of DNA extracted from the primary tumor tissues. Nick‐translated oncogene probes and oncogene inserts labeled to high specific activities were used. We observed a 5‐ to 10‐fold amplification of one or more of c‐myc, N‐myc, Ki‐ras and N‐ras oncogenes in 56% of the tumor tissue samples, with these oncogenes not being amplified in the peripheral blood cells of the same patients, L‐myc and H‐ras were not amplified in any of our samples. The oncogene amplifications seemed to be associated with advanced stages of squamous cell carcinomas, with the ras and myc family oncogenes being amplified in stages 3 and 4. Hybridization with N‐myc detected an additional 2.3 kb EcoRI fragment, along with the normal 2.1 kb fragment. Our data also demonstrated amplification of multiple oncogenes in the same tumor tissue sample. About 60% of the samples with amplified oncogenes showed simultaneous amplification of 2 or more oncogenes. The results showing different oncogene amplifications in similar tumors, as well as multiple oncogene amplifications in the same tumor, suggest that these oncogenes may be alternatively or simultaneously activated in oral carcinogenesis.Keywords
This publication has 35 references indexed in Scilit:
- myc oncogenes: activation and amplificationBiochimica et Biophysica Acta (BBA) - Reviews on Cancer, 1987
- The expression of oncogenes in human developing liver and hepatomasBiochemical and Biophysical Research Communications, 1987
- Association of Multiple Copies of the N-mycOncogene with Rapid Progression of NeuroblastomasNew England Journal of Medicine, 1985
- Evolution of tumours and the impact of molecular oncologyNature, 1985
- Oncogenes: Their presence and significance in squamous cell cancer of the head and neckThe Laryngoscope, 1985
- Amplification of N- myc in Untreated Human Neuroblastomas Correlates with Advanced Disease StageScience, 1984
- Isolation of a transforming sequence from a human bladder carcinoma cell lineCell, 1982
- Experimental studies on betel nut and tobacco carcinogenicityInternational Journal of Cancer, 1976
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975