Patterns and Dynamics of Subventricular Zone Neuroblast Migration in the Ischemic Striatum of the Adult Mouse
Open Access
- 13 May 2009
- journal article
- Published by SAGE Publications in Journal of Cerebral Blood Flow & Metabolism
- Vol. 29 (7), 1240-1250
- https://doi.org/10.1038/jcbfm.2009.55
Abstract
The migratory behavior of neuroblasts after a stroke is poorly understood. Using time-lapse microscopy, we imaged migration of neuroblasts and cerebral vessels in living brain slices of adult doublecortin (DCX, a marker of neuroblasts) enhanced green fluorescent protein (eGFP) transgenic mice that were subjected to 7 days of stroke. Our results show that neuroblasts originating in the subventricular zone (SVZ) of adult mouse brain laterally migrated in chains or individually to reach the ischemic striatum. The chains were initially formed at the border between the SVZ and the striatum by neuroblasts in the SVZ and then extended to the striatum. The average speed of DCX-eGFP-expressing cells within chains was 28.67 ± 1.04 μm/h, which was significantly faster ( P < 0.01) than the speed of the cells in the SVZ (17.98 ± 0.57 μm/h). Within the ischemic striatum, individual neuroblasts actively extended or retracted their processes, suggestive of probing the immediate microenvironment. The neuroblasts close to cerebral blood vessels exhibited multiple processes. Our data suggest that neuroblasts actively interact with the microenvironment to reach the ischemic striatum by multiple migratory routes.Keywords
This publication has 46 references indexed in Scilit:
- Long-Term Neuroblast Migration Along Blood Vessels in an Area With Transient Angiogenesis and Increased Vascularization After StrokeStroke, 2007
- Multiphoton microscope imaging: The behavior of neural progenitor cells in the rostral migratory streamNeuroscience Letters, 2007
- Dynamic features of postnatal subventricular zone cell motility: A two‐photon time‐lapse studyJournal of Comparative Neurology, 2007
- Subventricular Zone Stem Cells Are Heterogeneous with Respect to Their Embryonic Origins and Neurogenic Fates in the Adult Olfactory BulbJournal of Neuroscience, 2007
- Doublecortin is necessary for the migration of adult subventricular zone cells from neurospheresMolecular and Cellular Neuroscience, 2006
- Subventricular Zone-Derived Neuroblasts Migrate and Differentiate into Mature Neurons in the Post-Stroke Adult StriatumJournal of Neuroscience, 2006
- Matrix Metalloproteinase 2 (MMP2) and MMP9 Secreted by Erythropoietin-Activated Endothelial Cells Promote Neural Progenitor Cell MigrationJournal of Neuroscience, 2006
- Doublecortin maintains bipolar shape and nuclear translocation during migration in the adult forebrainNature Neuroscience, 2006
- Involvement of Matrix Metalloproteinase in Neuroblast Cell Migration from the Subventricular Zone after StrokeJournal of Neuroscience, 2006
- Stromal Cell-Derived Factor 1α Mediates Neural Progenitor Cell Motility after Focal Cerebral IschemiaJournal of Cerebral Blood Flow & Metabolism, 2005