Gene expression profile of human endometrial receptivity: comparison between natural and stimulated cycles for the same patients
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Open Access
- 26 February 2009
- journal article
- research article
- Published by Oxford University Press (OUP) in Human Reproduction
- Vol. 24 (6), 1436-1445
- https://doi.org/10.1093/humrep/dep039
Abstract
The adjunction of exogenous hormones for controlled ovarian stimulation (COS) may alter endometrial receptiveness. In order to identify the genes misregulated under COS, we compared the endometrium gene expression profiles, from the same patients, in a natural cycle and in a subsequent COS cycle. For the same normal-responder patients (n = 21), endometrial biopsies (n = 84) were collected during the pre-receptive (LH + 2) and receptive stages (LH + 7) of a natural cycle and, subsequently, on oocyte retrieval day (hCG + 2) and on transfer day (hCG + 5) of a stimulated cycle. Samples were analyzed using DNA microarrays. Gene expression profiles and biological pathways involved in endometrial receptivity were analyzed. Although endometrium transition profiles from pre-receptive to receptive phases are similar between patients, COS regimens alter endometrial receptivity in comparison with natural cycle. Under COS conditions, two endometrial profiles were identified and were associated either with a moderately altered receptivity profile for the majority of the patients or a strongly altered profile for a sub-category of patients. The receptive endometrium transcription profile under COS was defective for biological functions such as TGFβ signaling, leukocyte transendothelial migration and the cell cycle. Gonadotrophin treatments in COS cycles led to disruptions of the transcriptional activation of genes involved in normal endometrial receptivity. We propose that when the receptiveness of the endometrium is seriously compromised by the COS protocol, fresh embryo replacement should be cancelled, the embryo frozen and thawed embryo replacement should be performed under natural cycles.Keywords
This publication has 37 references indexed in Scilit:
- Endometrial biopsy-induced gene modulation: first evidence for the expression of bladder-transmembranal uroplakin Ib in human endometriumFertility and Sterility, 2009
- Gene expression profiling of human peri-implantation endometria between natural and stimulated cyclesFertility and Sterility, 2008
- Local injury to the endometrium in controlled ovarian hyperstimulation cycles improves implantation ratesFertility and Sterility, 2008
- Regulation of DNA repair throughout the cell cycleNature Reviews Molecular Cell Biology, 2008
- A new method for class prediction based on signed-rank algorithms applied to Affymetrix® microarray experimentsBMC Bioinformatics, 2008
- Uterine Receptivity: Alterations Associated with Benign Gynecological DiseaseSeminars in Reproductive Medicine, 2007
- BABELOMICS: a systems biology perspective in the functional annotation of genome-scale experimentsNucleic Acids Research, 2006
- Platelet binding to monocytes increases the adhesive properties of monocytes by up-regulating the expression and functionality of β1 and β2 integrinsJournal of Leukocyte Biology, 2006
- Local injury to the endometrium doubles the incidence of successful pregnancies in patients undergoing in vitro fertilizationFertility and Sterility, 2003
- Significance analysis of microarrays applied to the ionizing radiation responseProceedings of the National Academy of Sciences of the United States of America, 2001