Prevalence of Pathogenic Escherichia coli in the Broiler House Environment
- 1 January 2004
- journal article
- Published by American Association of Avian Pathologists (AAAP) in Avian Diseases
- Vol. 48 (1), 189-195
- https://doi.org/10.1637/7043
Abstract
SUMMARY. Matched sampling of Escherichia coli from broiler house litter and bird lesions of either cellulitis or colibacillosis was conducted to investigate the relationship of pathogenic E. coli to those found in the environment. Isolates were collected from six broiler flocks representing six geographically disparate ranches. Isolates were compared by flock for similarity in serotype and genotyped by pulsed-field gel electrophoresis. Serotyping revealed a considerable dissociation between the two groups of isolates. The prevalence of pathogenic E. coli that matched the environmental isolates from the same house was 0 to 3%. Statistical analysis of the serotype data showed a strong dependence of serotype on isolate source, indicating a high probability that a particular serotype would be found among lesions or litter but not in both groups. Genotyping of isolates on two farms supported the results of serotyping and provided differentiation of isolates that could not by typed by serology. These results suggested that the prevalence of pathogenic E. coli in the broiler house was independent of the prevalence of other commensal or environmental E. coli. Understanding the composition of E. coli populations in commercial poultry production may have bearing on the epidemiology and control of E. coli related diseases. RESUMEN. Nota de Investigacion—Prevalencia de Escherichia coli patogeno en el medioKeywords
This publication has 9 references indexed in Scilit:
- Virulence Factors of Escherichia coli from Cellulitis or Colisepticemia Lesions in ChickensAvian Diseases, 2002
- Persistence of cellulitis-associated Escherichia coli DNA fingerprints in successive broiler chicken flocksVeterinary Microbiology, 2000
- A Statistical Model for Assessing Sample Size for Bacterial Colony Selection: A Case Study of Escherichia Coli and Avian CellulitisJournal of Veterinary Diagnostic Investigation, 2000
- Cloning and sequencing of the iss gene from a virulent avian Escherichia coli.Avian Diseases, 2000
- Spatial heterogeneity of Escherichia coli DNA fingerprints isolated from cellulitis lesions in chickens.Avian Diseases, 1999
- Assessing cellulitis pathogenicity of Escherichia coli isolates in broiler chickens assessed by an in vivo inoculation model.Avian Diseases, 1999
- A Reproducible Model for the Induction of Avian Cellulitis in Broiler ChickensAvian Diseases, 1997
- Clonal relationships and variation in virulence among Escherichia coli strains of avian originMicrobial Pathogenesis, 1993
- Escherichia coli Infection in PoultryResearch in Veterinary Science, 1961