Effects of a prolonged standardized diet on normalizing the human metabolome
- 1 December 2009
- journal article
- Published by Elsevier BV in The American Journal of Clinical Nutrition
- Vol. 90 (6), 1496-1501
- https://doi.org/10.3945/ajcn.2009.28234
Abstract
Background: Although the effects of acute dietary interventions on the human metabolome have been studied, the extent to which the metabolome can be normalized by extended dietary standardization has not yet been examined. Objective: We examined the metabolic profiles of healthy human subjects after extended dietary standardization to see whether the inherent variation in the human metabolome could be decreased. Design: A cohort of 10 healthy volunteers was admitted to a clinical research center for 2 wk of dietary standardization. Daily serum and urine samples and serum samples at a 2-wk follow-up visit were collected. The samples were analyzed by 1H nuclear magnetic resonance (NMR) spectroscopy and multivariate statistical analyses. Results: NMR spectra were collected to globally profile the higher-concentration metabolites (>μmol/L concentrations). Metabolic changes were observed in some serum samples after day 1 or the 2-wk follow-up visit. For each subject, the samples from all other days had similar profiles. The urinary metabolome reflected no effects from dietary standardization. Pooled 24-h urine samples were studied, which indicated that any normalization that does occur would do so in Conclusions: For both the urinary and serum metabolome, a single day of dietary standardization appears to provide all of the normalization that is achievable within the strict controls implemented in a clinical research setting. After 24 h, the subjects remain in their metabolic space; the remaining intra- and intersubject variations appear to be influenced by variables such as genetics, age, and lifestyle.Funding Information
- National Institutes of Health (GM38147, P30ES10126, RR000046)
This publication has 19 references indexed in Scilit:
- Mouse population-guided resequencing reveals that variants in CD44 contribute to acetaminophen-induced liver injury in humansGenome Research, 2009
- Evidence of different metabolic phenotypes in humansProceedings of the National Academy of Sciences of the United States of America, 2008
- Nutritional Metabonomics: Applications and PerspectivesJournal of Proteome Research, 2007
- Susceptibility of Human Metabolic Phenotypes to Dietary ModulationJournal of Proteome Research, 2006
- Metabonomics in Toxicology: A ReviewToxicological Sciences, 2005
- Metabonomics, dietary influences and cultural differences: a 1H NMR-based study of urine samples obtained from healthy British and Swedish subjectsJournal of Pharmaceutical and Biomedical Analysis, 2004
- Understanding 'Global' Systems Biology: Metabonomics and the Continuum of MetabolismNature Reviews Drug Discovery, 2003
- Metabonomics: a platform for studying drug toxicity and gene functionNature Reviews Drug Discovery, 2002
- Combining Genomics, Metabolome Analysis, and Biochemical Modelling to Understand Metabolic NetworksComparative and Functional Genomics, 2001
- 'Metabonomics': understanding the metabolic responses of living systems to pathophysiological stimuli via multivariate statistical analysis of biological NMR spectroscopic dataXenobiotica, 1999