Virus Inactivation Mechanisms: Impact of Disinfectants on Virus Function and Structural Integrity
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- 26 October 2012
- journal article
- Published by American Chemical Society (ACS) in Environmental Science & Technology
- Vol. 46 (21), 12069-12078
- https://doi.org/10.1021/es3029473
Abstract
Oxidative processes are often harnessed as tools for pathogen disinfection. Although the pathways responsible for bacterial inactivation with various biocides are fairly well understood, virus inactivation mechanisms are often contradictory or equivocal. In this study, we provide a quantitative analysis of the total damage incurred by a model virus (bacteriophage MS2) upon inactivation induced by five common virucidal agents (heat, UV, hypochlorous acid, singlet oxygen, and chlorine dioxide). Each treatment targets one or more virus functions to achieve inactivation: UV, singlet oxygen, and hypochlorous acid treatments generally render the genome nonreplicable, whereas chlorine dioxide and heat inhibit host-cell recognition/binding. Using a combination of quantitative analytical tools, we identified unique patterns of molecular level modifications in the virus proteins or genome that lead to the inhibition of these functions and eventually inactivation. UV and chlorine treatments, for example, cause site-specific capsid protein backbone cleavage that inhibits viral genome injection into the host cell. Combined, these results will aid in developing better methods for combating waterborne and foodborne viral pathogens and further our understanding of the adaptive changes viruses undergo in response to natural and anthropogenic stressors.Keywords
This publication has 49 references indexed in Scilit:
- Oxidation of Virus Proteins during UV254 and Singlet Oxygen Mediated InactivationEnvironmental Science & Technology, 2010
- Mechanistic Aspects of Adenovirus Serotype 2 Inactivation with Free ChlorineApplied and Environmental Microbiology, 2010
- Inactivation of Adenoviruses, Enteroviruses, and Murine Norovirus in Water by Free Chlorine and MonochloramineApplied and Environmental Microbiology, 2010
- Detection of Oxidative Damages on Viral Capsid Protein for Evaluating Structural Integrity and Infectivity of Human NorovirusEnvironmental Science & Technology, 2009
- Mechanisms of Bacteriophage Inactivation via Singlet Oxygen Generation in UV Illuminated Fullerol SuspensionsEnvironmental Science & Technology, 2009
- Bleach Activates a Redox-Regulated Chaperone by Oxidative Protein UnfoldingCell, 2008
- Inactivation of Adenovirus Types 2, 5, and 41 in Drinking Water by UV Light, Free Chlorine, and MonochloramineJournal of Environmental Engineering, 2007
- Mechanisms of inactivation of hepatitis A virus in water by chlorine dioxideWater Research, 2004
- Capsid Functions of Inactivated Human Picornaviruses and Feline CalicivirusApplied and Environmental Microbiology, 2003
- Inactivation by chlorine of single poliovirus particles in waterEnvironmental Science & Technology, 1979