Driven by economic and time constraints, some medical centers and third parties are resterilizing single-use devices (SUDs) for reuse. The steam autoclave is quick, but most plastics used in SUDs cannot survive the temperature. Thus, a number of new methods of cleaning, disinfecting, and sterilizing these complex devices are being introduced on the market. The present study investigated the effects of a range of methods on the tensile strength of latex rubber, silicone elastomer, 2 different formulations of polyurethane, nylon, and high-density poly-ethylene (HDPE) specimens. The methods used were sodium hypochlorite bleach (Clorox™), peracetic acid + hydrogen peroxide (Steris™), formaldehyde gas (Chemiclave™), low-temperature peracetic acid and gas plasma (Plazlyte™), and low-temperature hydrogen peroxide gas plasma (Sterrad™). The results showed that silicone elastomer was minimally affected, whereas the strengths of nylon, polyethylene, and latex were reduced by some of the methods. Depending on the formulation, the strength of polyurethane either increased or decreased. The data demonstrated that disinfection and sterilization can affect the tensile strength of certain materials used in medical devices.