Expression in animal cells of the 5‐HT1A receptor by a vaccinia virus vector system
- 27 April 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 301 (3), 303-306
- https://doi.org/10.1016/0014-5793(92)80262-f
Abstract
The co-infection or infection—transfection variants of the T7 RNA polymerase/vaccinia vector system were used to express 5-HT1ARs in COS-7, BSC-40 and GH3 cells, with co-infection giving ca. 3-fold higher level than infection—transfection. Binding affinities were similar to those of the endogenous 5-HT1AR, with highest affinities for 5-HT and 8-OH-DPAT. Functional properties were demonstrated by assays of agonist-stimulated GTPase activity and its inhibition by pertussin toxin. Immunoblot assays showed expression of the unglycosylated and glycosylated receptor protein in the membrane and, surprisingly, in the cytosolic fractions.Keywords
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