Cell Fate in the Arabidopsis Root Epidermis Is Determined by Competition between WEREWOLF and CAPRICE
- 13 September 2011
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 157 (3), 1196-1208
- https://doi.org/10.1104/pp.111.185785
Abstract
The root hair and nonhair cells in the Arabidopsis (Arabidopsis thaliana) root epidermis are specified by a suite of transcriptional regulators. Two of these are WEREWOLF (WER) and CAPRICE (CPC), which encode MYB transcription factors that are required for promoting the nonhair cell fate and the hair cell fate, respectively. However, the precise function and relationship between these transcriptional regulators have not been fully defined experimentally. Here, we examine these issues by misexpressing the WER gene using the GAL4-upstream activation sequence transactivation system. We find that WER overexpression in the Arabidopsis root tip is sufficient to cause epidermal cells to adopt the nonhair cell fate through direct induction of GLABRA2 (GL2) gene expression. We also show that GLABRA3 (GL3) and ENHANCER OF GLABRA3 (EGL3), two closely related bHLH proteins, are required for the action of the overexpressed WER and that WER interacts with these bHLHs in plant cells. Furthermore, we find that CPC suppresses the WER overexpression phenotype quantitatively. These results show that WER acts together with GL3/EGL3 to induce GL2 expression and that WER and CPC compete with one another to define cell fates in the Arabidopsis root epidermis.Keywords
This publication has 50 references indexed in Scilit:
- Time to Stop: Flower Meristem TerminationPlant Physiology, 2009
- TheMYB23Gene Provides a Positive Feedback Loop for Cell Fate Specification in theArabidopsisRoot EpidermisTHE PLANT CELL ONLINE, 2009
- A Systems Approach Reveals Regulatory Circuitry for Arabidopsis Trichome Initiation by the GL3 and GL1 SelectorsPLoS Genetics, 2009
- Key Divisions in the Early Arabidopsis Embryo Require POL and PLL1 Phosphatases to Establish the Root Stem Cell Organizer and Vascular AxisDevelopmental Cell, 2008
- A competitive complex formation mechanism underlies trichome patterning on Arabidopsis leavesMolecular Systems Biology, 2008
- Distinct and overlapping roles of single-repeat MYB genes in root epidermal patterningDevelopmental Biology, 2007
- CONSTANS and the CCAAT Box Binding Complex Share a Functionally Important Domain and Interact to Regulate Flowering ofArabidopsisTHE PLANT CELL ONLINE, 2006
- Epidermal Cell Fate Determination in Arabidopsis : Patterns Defined by a Steroid-Inducible RegulatorScience, 1994
- A movable and regulable inactivation function within the steroid binding domain of the glucocorticoid receptorCell, 1988
- EMS- and relation-induced mutation frequencies at individual loci in Arabidopsis thaliana (L.) HeynhMutation Research - Reviews in Mutation Research, 1982