R9AP, a membrane anchor for the photoreceptor GTPase accelerating protein, RGS9-1
Open Access
- 15 July 2002
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 99 (15), 9755-9760
- https://doi.org/10.1073/pnas.152094799
Abstract
The regulator of G protein signaling (RGS)-9-1⋅Gβ5 complex forms the GTPase accelerating protein for Gαt in vertebrate photoreceptors. Although the complex is soluble when expressed in vitro, extraction of the endogenous protein from membranes requires detergents. The detergent extracts contain a complex of RGS9-1, Gβ5, Gαt, and a 25-kDa phosphoprotein, R9AP (RGS9-1-Anchor Protein). R9AP is encoded by one intronless gene in both human and mouse. Full or partial cDNA or genomic clones were obtained from mice, cattle, human, zebrafish, and Xenopus laevis. R9AP mRNA was detected only in the retina, and the protein only in photoreceptors. R9AP binds to the N-terminal domain of RGS9-1, and anchors it to the disk membrane via a C-terminal transmembrane helix.This publication has 49 references indexed in Scilit:
- Dependence of RGS9–1 Membrane Attachment on Its C-terminal TailPublished by Elsevier BV ,2001
- RGS9-Gβ5 Substrate Selectivity in PhotoreceptorsPublished by Elsevier BV ,2001
- Regulator of G Protein Signaling 8 (RGS8) Requires Its NH2 Terminus for Subcellular Localization and Acute Desensitization of G Protein-gated K+ ChannelsPublished by Elsevier BV ,2001
- Modules in the Photoreceptor RGS9-1·Gβ5L GTPase-accelerating Protein Complex Control Effector Coupling, GTPase Acceleration, Protein Folding, and StabilityPublished by Elsevier BV ,2000
- Protein secondary structure prediction based on position-specific scoring matrices 1 1Edited by G. Von HeijneJournal of Molecular Biology, 1999
- A GTPase-accelerating factor for transducin, distinct from its effector cGMP phosphodiesterase, in rod outer segment membranesNeuron, 1993
- Amplification and kinetics of the activation steps in phototransductionBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1993
- Membrane protein structure prediction: Hydrophobicity analysis and the positive-inside ruleJournal of Molecular Biology, 1992
- The effect of a single amino acid substitution on the antigenic specificity of the loop region of lysozymeMolecular Immunology, 1980
- The demonstration of two γ-globulin subclasses in the goatImmunochemistry, 1969