Serum protein concentrations from clinically healthy horses determined by agarose gel electrophoresis
- 26 February 2009
- journal article
- Published by Wiley in Veterinary Clinical Pathology
- Vol. 38 (1), 73-77
- https://doi.org/10.1111/j.1939-165x.2008.00100.x
Abstract
Serum protein electrophoresis is a useful screening test in equine laboratory medicine. The method can provide valuable information about changes in the concentrations of albumin and alpha-, beta-, and gamma-globulins and thereby help characterize dysproteinemias in equine patients. Reference values for horses using agarose gel as a support medium have not been reported.The purpose of this study was to establish reference intervals for serum protein concentrations in adult horses using agarose gel electrophoresis and to assess differences between warm-blooded and heavy draught horses. In addition, the precision of electrophoresis for determining fraction percentages and the detection limit were determined.Blood samples were obtained from 126 clinically healthy horses, including 105 Thoroughbreds and 21 heavy draught horses of both sexes and ranging from 2 to 20 years of age. The total protein concentration was determined by an automated biuret method. Serum protein electrophoresis was performed using a semi-automated agarose gel electrophoresis system. Coefficients of variation (CVs) were calculated for within-run and within-assay precision. Data from warm-blooded and draught horses were compared using the Mann-Whitney U test.Within-run and within-assay CVs were <5% for all protein fractions. No significant difference was found between warm-blooded and heavy draught horses and so combined reference intervals (2.5-97.5%) were calculated for total protein (51.0-72.0 g/L), albumin (29.6-38.5 g/L), alpha(1)-globulin (1.9-3.1 g/L), alpha(2)-globulin (5.3-8.7 g/L), beta(1)-globulin (2.8-7.3g/L), beta(2)-globulin (2.2-6.0 g/L), and gamma-globulin (5.8-12.7 g/L) concentrations, and albumin/globulin ratio (0.93-1.65).Using agarose gel as the supporting matrix for serum protein electrophoresis in horses resulted in excellent resolution and accurate results that facilitated standardization into 6 protein fractions.Keywords
This publication has 11 references indexed in Scilit:
- Evaluation of a commercially available human serum amyloid A (SAA) turbidometric immunoassay for determination of equine SAA concentrationsThe Veterinary Journal, 2006
- Serum protein electrophoresis in retired racing GreyhoundsVeterinary Clinical Pathology, 2005
- Serum Proteins and the DysproteinemiasPublished by Elsevier BV ,1997
- Evaluation of a Test Kit for Determination of Serum Immunoglobulin G Concentration in FoalsJournal of Veterinary Internal Medicine, 1988
- Serum protein electrophoresis in horses and poniesEquine Veterinary Journal, 1982
- A comparison of the serum protein electrophoretic patterns of young and adult animalsVeterinary Research Communications, 1981
- Species characteristics of serum proteins demonstrated after agarose gel electrophoresisVeterinary Research Communications, 1981
- A candidate reference method for determination of total protein in serum. I. Development and validation.Clinical Chemistry, 1981
- Agarose gel electrophoresis.Clinical Chemistry, 1979
- Serum Protein Fractions in Domestic AnimalsBritish Veterinary Journal, 1972