Characterisation of γ‐glutamylcysteine synthethase‐heavy subunit promoter: a critical role for AP‐1
- 1 May 1998
- journal article
- Published by Wiley in FEBS Letters
- Vol. 427 (1), 129-133
- https://doi.org/10.1016/s0014-5793(98)00410-4
Abstract
The 5′-flanking region of human γ-glutamylcysteine synthetase-heavy subunit (γ-GCS-HS) was characterised by creating a series of chloramphenicol acetyl transferase (CAT) reporter deletion constructs. Analysis of various deleted CAT constructs revealed that a putative AP-1 consensus sequence is required to direct the constitutive and oxidant-mediated promoter activity. Gel mobility shift and mutation analysis of the sequence (−269 to −263 bp), showed binding of AP-1 is involved in the oxidant-mediated regulation of γ-GCS-HS promoter activity.Keywords
This publication has 20 references indexed in Scilit:
- Tumor Necrosis Factor Increases Hepatocellular Glutathione by Transcriptional Regulation of the Heavy Subunit Chain of γ-Glutamylcysteine SynthetasePublished by Elsevier BV ,1997
- Proximal 5′-Flanking Sequence of the Human γ-Glutamylcysteine Synthetase Heavy Subunit Gene Is Involved in Cisplatin-Induced Transcriptional Up-regulation in a Lung Cancer Cell Line SBC-3Biochemical and Biophysical Research Communications, 1997
- Expression of Glutathione and γ-Glutamylcysteine Synthetase mRNA Is Jun DependentBiochemical and Biophysical Research Communications, 1997
- Constitutive and β-Naphthoflavone-induced Expression of the Human γ-Glutamylcysteine Synthetase Heavy Subunit Gene Is Regulated by a Distal Antioxidant Response Element/TRE SequencePublished by Elsevier BV ,1997
- Transcriptional Regulation of γ-Glutamylcysteine Synthetase-Heavy Subunit by Oxidants in Human Alveolar Epithelial CellsBiochemical and Biophysical Research Communications, 1996
- Induction of γ‐glutamylcysteine synthetase by cigarette smoke is associated with AP‐1 in human alveolar epithelial cellsFEBS Letters, 1996
- Identification of a Putative Antioxidant Response Element in the 5′-Flanking Region of the Human γ-Glutamylcysteine Synthetase Heavy Subunit GeneBiochemical and Biophysical Research Communications, 1995
- GSH1, which encodes gamma-glutamylcysteine synthetase, is a target gene for yAP-1 transcriptional regulation.Molecular and Cellular Biology, 1994
- Cooperative Interaction Between Ets and AP-1 Transcription Factors Regulates Induction of Glutathione S-Transferase Ya Gene ExpressionBiochemical and Biophysical Research Communications, 1994
- Glutathione deficiency and human immunodeficiency virus infectionThe Lancet, 1992