Evaluation of the extraction methods for arsenic speciation in rice straw, Oryza sativa L., and analysis by HPLC-HG-AFS
- 12 January 2005
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Journal of Analytical Atomic Spectrometry
- Vol. 20 (2), 103-110
- https://doi.org/10.1039/b416102e
Abstract
The most important and difficult step during arsenic speciation analysis is to extract the various arsenic species in the original forms from the samples. In this work, a study has been carried out to compare the different extraction methods by using different extraction solvents including water, methanol, water–methanol, water–ethanol and water–acetonitrile. Different techniques (shaking, ultrasonic, Soxhlet and microwave-assisted extraction) were applied during the procedure. A new, nontoxic and effective extraction method for arsenic speciation in the terrestrial plant material, rice straw, with water–ethanol mixture as extraction solvent using microwave-assisted extraction was found to provide good extraction efficiency without internal species conversion. The extraction of arsenic from the rice straw and subsequent determination of individual arsenic species by high performance liquid chromatography-hydride generation-atomic fluorescence spectrometry (HPLC-HG-AFS) is described. By using the proposed extraction method, the total arsenic extraction efficiency was 90.3%. The arsenic species in rice straw were analyzed and four normal chemical forms, AsIII, AsV, MMAV and DMAV, in straw shoots were found, but no MMAV was found in the straw roots.This publication has 55 references indexed in Scilit:
- Safety Evaluation of Organoarsenical Species in Edible Porphyra from the China SeaJournal of Agricultural and Food Chemistry, 2003
- Arsenic speciation in terrestrial plant material using microwave-assisted extraction, ion chromatography and inductively coupled plasma mass spectrometryJournal of Analytical Atomic Spectrometry, 2003
- Arsenic Accumulation and Metabolism in Rice (Oryza sativa L.)Environmental Science & Technology, 2002
- Comparison of enzymatic extraction procedures for use with directly coupled high performance liquid chromatography-inductively coupled plasma mass spectrometry for the speciation of arsenic in baby foodsAnalytica Chimica Acta, 2001
- Determination of total and speciated arsenic in rice by ion chromatography and inductively coupled plasma mass spectrometryJournal of Analytical Atomic Spectrometry, 2001
- Speciation of cationic arsenic species in seafood by coupling liquid chromatography with hydride generation atomic fluorescence detectionJournal of Analytical Atomic Spectrometry, 2000
- Coupled techniques based on liquid chromatography and atomic fluorescence detection for arsenic speciationAnalytica Chimica Acta, 2000
- Optimisation of the extraction of arsenic species from mussels with low power focused microwaves by applying a Doehlert designAnalytica Chimica Acta, 1998
- Determination of Arsenobetaine in Manufactured Seafood Products by Liquid Chromatography, Microwave-assisted Oxidation and Hydride Generation Atomic Absorption SpectrometryJournal of Analytical Atomic Spectrometry, 1997
- Arsenic speciation in biological samples by on-line high performance liquid chromatography-microwave digestionhydride generation-atomic absorption spectrometryAnalytica Chimica Acta, 1996